Cellosaurus cell line (CVCL

Cellosaurus (CVCL_C561)

Comments

Characteristics: Transposed with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein.
Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:25001; ZBTB44.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04486; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_ZJ91 (SEES3-1V)
Children:

CVCL_A8IG (3A1 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IH (3A2 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8II (3A6 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8IJ (3A7 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IK (3B1)	CVCL_A8IL (3B3 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8IM (3B4 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IN (3B6 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IP (3C2 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8IQ (3C4 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IR (3C6 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IS (3D1 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8IT (3D2 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IU (3D3 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IV (3D5 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8IW (3D7)	CVCL_F0SM (3D9 [Mouse hybridoma against NNK])	CVCL_A8IX (3E1 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8IY (3E4 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8IZ (3E5 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JA (3F1)
CVCL_A8JB (3F2 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JC (3F4 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JD (3F5 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8JE (3F6 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JF (3G1 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JG (3G2 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8JH (3G6 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JI (3G7)	CVCL_A8JJ (3H2 [Mouse hybridoma against SARS-CoV-2 spike])
CVCL_A8JK (3H4 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_A8JL (3H6)	CVCL_D3TZ (455-2A4)
CVCL_F0SN (4H2 [Mouse hybridoma against NNK])	CVCL_A8JM (5A1 [Mouse hybridoma against SARS-CoV-2 spike])	CVCL_F0WF (7G12 [Mouse hybridoma against NNK])
CVCL_F0WG (8G10 [Mouse hybridoma against NNK])	CVCL_A8JN (9F2)	CVCL_F0AD (anti-ULBP6/2/5)
CVCL_0F32 (IgG-13D3)	CVCL_0F33 (IgG-15A6)	CVCL_F1LF (Sp2/mIL-6 (hTERT))

Sex of cell

Male

Age at sampling

Blastocyst stage