Cellosaurus CCMB-Me-14-STGG-A1 (CVCL_C4SE)
| Cell line name | CCMB-Me-14-STGG-A1 |
|---|---|
| Synonyms | STGG |
| Accession | CVCL_C4SE |
| Resource Identification Initiative | To cite this cell line use: CCMB-Me-14-STGG-A1 (RRID:CVCL_C4SE) |
| Comments | Characteristics: Using CRISPR/Cas9 a T2A-H2B-tdTomato construct was inserted at the C-terminus of one allele of Sox2 and a H2B-GFP-SV40-Bsd cassette was inserted at the C-terminus of one allele of Gata6 (PubMed=36063726). Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Transfected with: HGNC; 4761; H2BC11. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: 129P2/Ola. |
| Hierarchy | Parent: CVCL_9108 (ES-E14TG2a) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=36063726; DOI=10.1016/j.scr.2022.102900 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q116048697 |
| Entry history | |
| Entry creation | 15-Dec-2022 |
| Last entry update | 29-Jun-2023 |
| Version number | 3 |