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Cellosaurus TH-mOrange hiPSC (CVCL_C3PH)

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Cell line name TH-mOrange hiPSC
Synonyms SP11#1 TH rep
Accession CVCL_C3PH
Resource Identification Initiative To cite this cell line use: TH-mOrange hiPSC (RRID:CVCL_C3PH)
Comments Part of: Spanish Stem Cell Bank (Banco Nacional de Lineas Celulares) collection.
From: Institut d'Investigacio Biomedica de Bellvitge (IDIBELL); Barcelona; Spain.
Characteristics: Using CRISPR/Cas9 P2A-mOrange construct was integrated after the stop codon of TH in both alleles (PubMed=31048719).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; G6HDH; mOrange (Note=Bright orange fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Skin, epidermis; UBERON=UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RI59 (SP11.1)
Sex of cell Female
Age at sampling 48Y
Category Induced pluripotent stem cell
STR profile Source(s): BNLC

Markers:
AmelogeninX
CSF1PO11,12
D2S133823,24
D3S135814,18
D5S81812
D7S8208,11
D8S117913,14
D13S31711,13
D16S53911,12
D18S5114,17
D19S43314,15
D21S1128,30
FGA20,24
TH019,10
TPOX10,11
vWA16

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Web pages Provider; BNLC; -; https://www.isciii.es/documents/20119/0/ANEXO+2_SP11-wild+type+TH+rep.pdf
Provider; BNLC; -; https://www.isciii.es/documents/20119/880364/Documento_Deposito_SP11-wild+type+TH-rep.pdf
Publications

PubMed=31048719; DOI=10.1038/s41598-019-43080-2; PMCID=PMC6497635
Carles Calatayud Aristoy, Giulia Carola, Irene Fernandez-Carasa, Marco Valtorta, Senda Jimenez-Delgado, Monica Diaz, Jordi Soriano-Fradera, Graziella Cappelletti, Javier Garcia-Sancho, Angel Raya, Antonella Consiglio;
CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.
Sci. Rep. 9:6811.1-6811.9(2019)

Cross-references
Encyclopedic resources Wikidata; Q116050651
Entry history
Entry creation15-Dec-2022
Last entry update10-Apr-2025
Version number6