Cellosaurus cell line MSHRIe002-A-SOX17 control 2 (CVCL

Cellosaurus MSHRIe002-A-SOX17 control 2 (CVCL_C3L7)

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Cell line name

MSHRIe002-A-SOX17 control 2

Synonyms

CA2 NoCre Sox17E

Accession

CVCL_C3L7

Resource Identification Initiative

To cite this cell line use: MSHRIe002-A-SOX17 control 2 (RRID:CVCL_C3L7)

Comments

Characteristics: Transfected with a loxP-BetaGeo(lacZ-neo)-polyA-loxP-SOX17-IRES-PuroR-polyA construct. This control cell line can be selected via neomycin resistance and does not overexpress SOX17 as this would require Cre-mediated excision of the loxP flanked betaGeo (PubMed=18682240).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:18122; SOX17.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_B845 (MSHRIe002-A)
Children:

CVCL_C3L9 (MSHRIe002-A-SOX17 overexpressing 2)

Sex of cell

Male

Age at sampling

Blastocyst stage