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Cellosaurus U2OS BFP-LMNB1 RFP-ACTB (CVCL_C3CB)

[Text version]
Cell line name U2OS BFP-LMNB1 RFP-ACTB
Accession CVCL_C3CB
Resource Identification Initiative To cite this cell line use: U2OS BFP-LMNB1 RFP-ACTB (RRID:CVCL_C3CB)
Comments Population: Caucasian.
Characteristics: Edited by ZFN at the LMNB1 locus to insert a TagBFP construct at the N-terminus of the gene and at the ACTB locus to insert a TagRFP construct at the N-terminus of the gene.
Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the BFP mTagBFP).
Transfected with: UniProtKB; D0VX33; Entacmaea quadricolor FP480 (with p.Thr6Lys, p.Gln41Met, p.His67Ser, p.Gln134Lys, p.Ala142Pro, p.His143Ser, p.Met146Lys, p.Tyr148Lys, p.Ala158Val, p.Leu160Met, p.Tyr197Arg, p.Arg201Ile, p.Val211Trp and p.Cys222Ser = TagRFP).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Children:
CVCL_C3C9 (U2OS BFP-LMNB1 GFP-TUBA1B RFP-ACTB)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Web pages https://www.sigmaaldrich.com/catalog/product/sigma/cll1038
Cross-references
Encyclopedic resources Wikidata; Q114313147
Entry history
Entry creation22-Sep-2022
Last entry update05-Oct-2023
Version number5