Cellosaurus cell line U251-NF-kappaB-GFP-Luc-A1 (CVCL

Cellosaurus U251-NF-kappaB-GFP-Luc-A1 (CVCL_C1CP)

Cross-references
Cell line name	U251-NF-kappaB-GFP-Luc-A1
Accession	CVCL_C1CP
Resource Identification Initiative	To cite this cell line use: U251-NF-kappaB-GFP-Luc-A1 (RRID:CVCL_C1CP)
Comments	Population: Caucasian. Characteristics: Transduced with a construct containing a fused GFP-Luc protein expressed under the control of a promoter containing multiple copies of the NF-kappaB response element. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase. Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Sequence variations	Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
Disease	Astrocytoma (NCIt: C60781) Astrocytoma (ORDO: Orphanet_94)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0021 (U-251MG)
Sex of cell	Male
Age at sampling	75Y
Category	Cancer cell line
Publications	PubMed=28790798; DOI=10.1155/2017/6209865; PMCID=PMC5534271 Marie-Therese Zeuner, Thomas Vallance, Sakthivel Vaiyapuri, Graeme Stuart Cottrell, Darius Widera; Development and characterisation of a novel NF-kappaB reporter cell line for investigation of neuroinflammation. Mediators Inflamm. 2017:6209865.1-6209865.10(2017)
Cell line databases/resources	cancercelllines; CVCL_C1CP
Encyclopedic resources	Wikidata; Q114313145
Entry history
Entry creation	22-Sep-2022
Last entry update	14-Aug-2025
Version number	9