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Cellosaurus CHO-K1-CNG-Gs (CVCL_C0RV)

[Text version]
Cell line name CHO-K1-CNG-Gs
Synonyms CHO-K1-CNG
Accession CVCL_C0RV
Resource Identification Initiative To cite this cell line use: CHO-K1-CNG-Gs (RRID:CVCL_C0RV)
Comments Characteristics: Expresses a modified cyclic nucleotide gated (CNG) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. Allows sensitive detection of signaling by Gs, Gi or Gq-coupled receptors (Dexorgen).
Characteristics: Expresses a constitutively active over-expressed Gs-coupled GPCR. Serves as a parent cell line for various ACTOne PDE cell lines.
Genetic integration: Method=Transduction; Gene=RGD; 2367; Cnga2 (Note=With p.Phe68Ala, p.Trp81Ala, p.Cys460Trp and p.Glu583Met = oCNG-NC).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Miscellaneous: Has Dexorgen catalog number CB-81300-100, but not distributed separately from children cell lines.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Children:
CVCL_C0U2 (ACTOne cAMP-PDE4A)CVCL_C0U3 (ACTOne cAMP-PDE4B)CVCL_C0U4 (ACTOne cAMP-PDE4C)
CVCL_C0U5 (ACTOne cAMP-PDE4D)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Cross-references
Encyclopedic resources Wikidata; Q112929380
Entry history
Entry creation23-Jun-2022
Last entry update10-Apr-2025
Version number7