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Cellosaurus SRD-15/pInsig-2 (CVCL_B7PF)

[Text version]
Cell line name SRD-15/pInsig-2
Accession CVCL_B7PF
Resource Identification Initiative To cite this cell line use: SRD-15/pInsig-2 (RRID:CVCL_B7PF)
Comments Characteristics: Transfected with INSIG2 followed by 6 tandem copies of the Myc epitope (PubMed=15866869).
Selected for resistance to: ChEBI; CHEBI_77317; 4-[2,2-bis(diethoxyphosphoryl)ethenyl]-2,6-ditert-butylphenol] (SR12813).
Selected for resistance to: ChEBI; CHEBI_42977; 25-hydroxycholesterol.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:20452; INSIG2.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCIt; C129643; Cesium-137 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q8CFA6; Insig1; Zygosity=Heterozygous; Note=The 2nd allele has a loss of function (from parent cell line).
  • Gene deletion; UniProtKB; Q8CFA5; Insig2; Zygosity=Heterozygous (PubMed=15866869).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_B7PD (SRD-15)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=15866869; DOI=10.1074/jbc.M502989200
Peter C.W. Lee, Navdar Sever, Russell A. DeBose-Boyd;
Isolation of sterol-resistant Chinese hamster ovary cells with genetic deficiencies in both Insig-1 and Insig-2.
J. Biol. Chem. 280:25242-25249(2005)

Cross-references
Encyclopedic resources Wikidata; Q112930422
Entry history
Entry creation23-Jun-2022
Last entry update14-Aug-2025
Version number9