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Cellosaurus MCF-7/PacR (CVCL_B7P7)

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Cell line name MCF-7/PacR
Synonyms MCF7/PacR; MCF-7/PAC
Accession CVCL_B7P7
Resource Identification Initiative To cite this cell line use: MCF-7/PacR (RRID:CVCL_B7P7)
Comments Population: Caucasian.
Characteristics: Generated by step-increasing drug concentration to 300 nm of paclitaxel. Overexpresses ABCB1 which is amplified and inserted in chromosome 3 (PubMed=27664577; Direct_author_submission).
Doubling time: ~25-30 hours (Direct_author_submission).
Selected for resistance to: ChEBI; CHEBI_45863; Paclitaxel (Taxol).
Omics: Proteomics; Subcellular; Mitochondrion; 2DE-MS.
Miscellaneous: Doubling time, ABCB1 status and STR profile from personal communication of Daniel, Petr.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX
CSF1PO10
D2S133821,23
D3S135816
D5S81811,12
D7S8209
D8S117910,14
D13S31711
D16S53911,12
D18S5114
D19S43313,14
D21S1130
FGA23,25
TH016
TPOX9,12
vWA14,15

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Publications

PubMed=27664577; DOI=10.1016/j.taap.2016.09.020
Vlasta Nemcova-Furstova, Dana Kopperova, Kamila Balusikova, Marie Ehrlichova, Veronika Brynychova, Radka Vaclavikova, Petr Daniel, Pavel Soucek, Jan Kovar;
Characterization of acquired paclitaxel resistance of breast cancer cells and involvement of ABC transporters.
Toxicol. Appl. Pharmacol. 310:215-228(2016)

PubMed=29625142; DOI=10.1016/j.taap.2018.04.002; PMCID=PMC5937276
Michael Jelinek, Kamila Balusikova, Petr Daniel, Vlasta Nemcova-Furstova, Palani Kirubakaran, Martin Jacek, Long-Fei Wei, Xin Wang, Jiri Vondrasek, Iwao Ojima, Jan Kovar;
Substituents at the C3' and C3'N positions are critical for taxanes to overcome acquired resistance of cancer cells to paclitaxel.
Toxicol. Appl. Pharmacol. 347:79-91(2018)

PubMed=31248089; DOI=10.3390/ijms20122986; PMCID=PMC6628585
Petr Daniel, Petr Halada, Michael Jelinek, Kamila Balusikova, Jan Kovar;
Differentially expressed mitochondrial proteins in human MCF7 breast cancer cells resistant to paclitaxel.
Int. J. Mol. Sci. 20:2986.1-2986.20(2019)

Cross-references
Cell line databases/resources cancercelllines; CVCL_B7P7
Encyclopedic resources Wikidata; Q112930090
Entry history
Entry creation23-Jun-2022
Last entry update10-Apr-2025
Version number8