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Cellosaurus Col-GFP HSC (CVCL_B7MI)

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Cell line name Col-GFP HSC
Synonyms Col-GFP-HSC; HSC Col-GFP
Accession CVCL_B7MI
Resource Identification Initiative To cite this cell line use: Col-GFP HSC (RRID:CVCL_B7MI)
Comments Doubling time: ~16 hours (DSMZ=ACC-941).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Miscellaneous: Age/sex of donor from personal communication of Weiskirchen, Ralf.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Hepatic stellate cell; CL=CL_0000632.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: pCol9GFP-HS4,5 transgenic.
Sex of cell Male
Age at sampling 6W
Category Transformed cell line
STR profile Source(s): PubMed=36067186

Markers:
Mouse STR 1-116
Mouse STR 1-219
Mouse STR 2-115
Mouse STR 3-214
Mouse STR 4-220.3
Mouse STR 5-517
Mouse STR 6-418
Mouse STR 6-717
Mouse STR 7-126.2,27.2
Mouse STR 8-116
Mouse STR 11-216
Mouse STR 12-117
Mouse STR 13-117
Mouse STR 15-322.3
Mouse STR 17-215
Mouse STR 18-316,17
Mouse STR 19-213
Mouse STR X-127

Run an STR similarity search on this cell line
Publications

PubMed=23437087; DOI=10.1371/journal.pone.0056116; PMCID=PMC3577806
Steffen K. Meurer, Muhammad Alsamman, Hacer Sahin, Hermann E. Wasmuth, Tatiana Kisseleva, David Allen Brenner, Christian Trautwein, Ralf Weiskirchen, David Scholten;
Overexpression of endoglin modulates TGF-beta1-signalling pathways in a novel immortalized mouse hepatic stellate cell line.
PLoS ONE 8:e56116.1-e56116.14(2013)

PubMed=36067186; DOI=10.1371/journal.pone.0274219; PMCID=PMC9447916
Steffen K. Meurer, David Allen Brenner, Ralf Weiskirchen;
Multiplex short tandem repeat profiling of immortalized hepatic stellate cell line Col-GFP HSC.
PLoS ONE 17:e0274219.1-e0274219.8(2022)

Cross-references
Cell line collections (Providers) DSMZ; ACC-941
Encyclopedic resources Wikidata; Q112929403
Entry history
Entry creation23-Jun-2022
Last entry update14-Aug-2025
Version number9