Cellosaurus logo
expasy logo

Cellosaurus U-2 OS-CRISPR-NUP96-mMaple clone no.16 (CVCL_B7FK)

[Text version]
Cell line name U-2 OS-CRISPR-NUP96-mMaple clone no.16
Accession CVCL_B7FK
Resource Identification Initiative To cite this cell line use: U-2 OS-CRISPR-NUP96-mMaple clone no.16 (RRID:CVCL_B7FK)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9D10A NUP96 has been endogenously tagged in both alleles at the C-terminus with mMaple (PubMed=31562488).
Transfected with: UniProtKB; Q9U6Y3; GFP-like fluorescent chromoprotein cFP484 (with many modification leading to the photoconvertible protein mMaple).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=31562488; DOI=10.1038/s41592-019-0574-9
Thevathasan J.V., Kahnwald M., Cieslinski K., Hoess P., Peneti S.K., Reitberger M., Heid D., Kasuba K.C., Hoerner S.J., Li Y.-M., Wu Y.-L., Mund M., Matti U., Pereira P.M., Henriques R., Nijmeijer B., Kueblbeck M., Sabinina V.J., Ellenberg J., Ries J.
Nuclear pores as versatile reference standards for quantitative superresolution microscopy.
Nat. Methods 16:1045-1053(2019)

Cross-references
Cell line collections (Providers) CLS; 300461
Encyclopedic resources Wikidata; Q112930491
Entry history
Entry creation23-Jun-2022
Last entry update05-Oct-2023
Version number5