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Cellosaurus U-2 OS-CRISPR-NUP96-mEGFP clone no.195 (CVCL_B7FJ)

[Text version]
Cell line name U-2 OS-CRISPR-NUP96-mEGFP clone no.195
Accession CVCL_B7FJ
Resource Identification Initiative To cite this cell line use: U-2 OS-CRISPR-NUP96-mEGFP clone no.195 (RRID:CVCL_B7FJ)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9D10A NUP96 has been endogenously tagged in both alleles at the C-terminus with mEGFP (PubMed=31562488).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=31562488; DOI=10.1038/s41592-019-0574-9
Thevathasan J.V., Kahnwald M., Cieslinski K., Hoess P., Peneti S.K., Reitberger M., Heid D., Kasuba K.C., Hoerner S.J., Li Y.-M., Wu Y.-L., Mund M., Matti U., Pereira P.M., Henriques R., Nijmeijer B., Kueblbeck M., Sabinina V.J., Ellenberg J., Ries J.
Nuclear pores as versatile reference standards for quantitative superresolution microscopy.
Nat. Methods 16:1045-1053(2019)

Cross-references
Cell line collections (Providers) CLS; 300174
Encyclopedic resources Wikidata; Q112930490
Entry history
Entry creation23-Jun-2022
Last entry update05-Oct-2023
Version number5