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Cellosaurus U-2 OS-CRISPR-NUP96-mEGFP clone no.195 (CVCL_B7FJ)

[Text version]
Cell line name U-2 OS-CRISPR-NUP96-mEGFP clone no.195
Accession CVCL_B7FJ
Resource Identification Initiative To cite this cell line use: U-2 OS-CRISPR-NUP96-mEGFP clone no.195 (RRID:CVCL_B7FJ)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9D10A NUP96 has been endogenously tagged in both alleles at the C-terminus with mEGFP (PubMed=31562488).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=31562488; DOI=10.1038/s41592-019-0574-9; PMCID=PMC6768092
Jervis Vermal Thevathasan, Maurice Kahnwald, Konstanty Cieslinski, Philipp Hoess, Sudheer Kumar Peneti, Manuel Reitberger, Daniel Heid, Krishna Chaitanya Kasuba, Sarah Janice Hoerner, Yi-Ming Li, Yu-Le Wu ...Show all 20 authors... , Markus Mund, Ulf Matti, Pedro Matos Pereira, Ricardo Henriques, Bianca Nijmeijer-Winter, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jan Ellenberg, Jonas Ries; Show fewer authors
Nuclear pores as versatile reference standards for quantitative superresolution microscopy.
Nat. Methods 16:1045-1053(2019)

Cross-references
Cell line collections (Providers) CLS; 300174
Encyclopedic resources Wikidata; Q112930490
Entry history
Entry creation23-Jun-2022
Last entry update10-Apr-2025
Version number8