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Cellosaurus MHHi001-A-9 (CVCL_B6RL)

[Text version]
Cell line name MHHi001-A-9
Synonyms hiPSCRCaMP1h/Neo
Accession CVCL_B6RL
Resource Identification Initiative To cite this cell line use: MHHi001-A-9 (RRID:CVCL_B6RL)
Comments From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
Population: Caucasian.
Characteristics: Using TALEN a CAG-promoter driven RCaMP1h and a pMYH6-Neo-pPGKp-HygroR construct were introduced in both alleles of the AAVS1 safe harbor locus (PubMed=35152180).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=TALEN; Gene=Entacmaea quadricolor RCaMP1h (Note=Red fluorescent calcium sensor).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_QX51 (MHHi001-A)
Sex of cell Female
Age at sampling <1D
Category Induced pluripotent stem cell
Publications

PubMed=35152180; DOI=10.1016/j.scr.2022.102697
Maria Elena Ricci Signorini, Monika Szepes, Anna Melchert, Mine Bakar, Sylvia Merkert, Alexandra Haase, Gudrun Gohring, Ulrich Martin, Ina Gruh;
Generation of human induced pluripotent stem cell lines encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f.
Stem Cell Res. 60:102697-102697(2022)

Cross-references
Cell line databases/resources hPSCreg; MHHi001-A-9
Encyclopedic resources Wikidata; Q112129317
Entry history
Entry creation17-Mar-2022
Last entry update14-Aug-2025
Version number8