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Cellosaurus iKLF1.12 (CVCL_B6RE)

[Text version]
Cell line name iKLF1.12
Accession CVCL_B6RE
Resource Identification Initiative To cite this cell line use: iKLF1.12 (RRID:CVCL_B6RE)
Comments Characteristics: Using ZFN a CAG-HA-KLF1-ERT2 construct has been inserted at the AAVS1 safe harbor locus so as to allow temporally controlled activation during macrophage differentiation.
Genetic integration: Method=ZFN; Gene=HGNC; HGNC:3467; ESR1 (Note=Ligand-binding domain; with p.Gly400Val, p.Met543Ala and p.Leu544Ala = ET[T2]).
Genetic integration: Method=ZFN; Gene=HGNC; HGNC:6345; KLF1.
Genetic integration: Method=ZFN; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UL47 (SFCi55)
Sex of cell Female
Category Induced pluripotent stem cell
Publications

PubMed=30787325; DOI=10.1038/s41467-019-08705-0; PMCID=PMC6382809
Martha Beatriz Lopez-Yrigoyen, Cheng-Tao Yang, Antonella Fidanza, Luca Cassetta, A. Helen Taylor, Angela McCahill, Erica Sellink, Marieke von Lindern, Emile van den Akker, Joanne C. Mountford, Jeffrey William Pollard, Lesley M. Forrester;
Genetic programming of macrophages generates an in vitro model for the human erythroid island niche.
Nat. Commun. 10:881.1-881.11(2019)

Cross-references
Encyclopedic resources Wikidata; Q111733492
Entry history
Entry creation17-Mar-2022
Last entry update14-Aug-2025
Version number7