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Cellosaurus Veggie-CHO:FLT-3L (CVCL_B6FI)

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Cell line name Veggie-CHO:FLT-3L
Accession CVCL_B6FI
Resource Identification Initiative To cite this cell line use: Veggie-CHO:FLT-3L (RRID:CVCL_B6FI)
Comments Group: Serum/protein free medium cell line.
Doubling time: 21.2 +- 2.9 hours (PubMed=19003404).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3766; FLT3LG.
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_B6FH (Veggie-CHO)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=19003404; DOI=10.1023/A:1008052908496; PMCID=PMC3449829
Brian Rasmussen, Ray Davis, James Thomas, Pranhitha Reddy;
Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line.
Cytotechnology 28:31-42(1998)

Cross-references
Encyclopedic resources Wikidata; Q112041912
Entry history
Entry creation17-Mar-2022
Last entry update19-Dec-2024
Version number7