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Cellosaurus Sf-RVNLec1 (CVCL_B5EE)

[Text version]
Cell line name Sf-RVNLec1
Synonyms SfMgat1t4#
Accession CVCL_B5EE
Resource Identification Initiative To cite this cell line use: Sf-RVNLec1 (RRID:CVCL_B5EE)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Characteristics: Produces immature, relatively minimally processed N-glycan precursors that can be removed from the polypeptide backbone using endo-beta-N-acetylglucosaminidase H (PubMed=34838817).
Virology: Devoid of SfRV rhabdovirus (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; UniProtKB; H2BEB9; Mgat1.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Species of origin Spodoptera frugiperda (Fall armyworm) (NCBI Taxonomy: 7108)
Hierarchy Parent: CVCL_JF76 (Sf-RVN)
Sex of cell Female
Age at sampling Pupal stage
Category Spontaneously immortalized cell line

PubMed=34838817; DOI=10.1016/j.jbc.2021.101454
Mabashi-Asazuma H., Jarvis D.L.
A new insect cell line engineered to produce recombinant glycoproteins with cleavable N-glycans.
J. Biol. Chem. 298:101454.1-101454.13(2022)

Encyclopedic resources Wikidata; Q110433215
Entry history
Entry creation16-Dec-2021
Last entry update29-Jun-2023
Version number5