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Cellosaurus M4A4 GFP (CVCL_B058)

[Text version]
Cell line name M4A4 GFP
Synonyms M4A4-GFP; M-4A4-GFP
Accession CVCL_B058
Resource Identification Initiative To cite this cell line use: M4A4 GFP (RRID:CVCL_B058)
Comments Problematic cell line: Contaminated. Grand-parent cell line (MDA-MB-435) has been shown to be a M14 derivative.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00561.
Population: Caucasian.
Transfected with: UniProtKB; P42212; GFP.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B057 (M4A4)
Children:
CVCL_B059 (M4A4 LM3-2 GFP)CVCL_B060 (M4A4 LM3-4 CL16 GFP)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO11
D5S81811,12
D7S8208,10
D13S31712
D16S53913
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://www.atcc.org/en/support/technical-support/faqs/atcc-htb-129-derivative
https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=14506175
Goodison S., Kawai K., Hihara J., Jiang P., Yang M., Urquidi V., Hoffman R.M., Tarin D.
Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein.
Clin. Cancer Res. 9:3808-3814(2003)

Cross-references
Cell line collections (Providers) ATCC; CRL-2915
Cell line databases/resources cancercelllines; CVCL_B058
Encyclopedic resources Wikidata; Q54903623
Entry history
Entry creation06-Jun-2012
Last entry update05-Oct-2023
Version number26