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Cellosaurus LUHMES (CVCL_B056)

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Cell line name LUHMES
Synonyms LUnd Human MESencephalic
Accession CVCL_B056
Resource Identification Initiative To cite this cell line use: LUHMES (RRID:CVCL_B056)
Comments Characteristics: Embryonic neuronal precursor cells that can be maintained as proliferating cells due to the expression of a tetracycline-regulatable (Tet-Off) v-myc transgene. Can be differentiated to post-mitotic neurons by the addition of tetracycline, GDNF and dibutyryl cAMP.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P01110; Avian myelocytomatosis virus 29 v-Myc.
Transformant: v-Myc.
Derived from site: In situ; Fetal brain, ventral mesencephalon; UBERON=UBERON_0001943.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B061 (MESC2.10)
Children:
CVCL_D2PI (LUHMES IDS KO clone 13)CVCL_D2PJ (LUHMES IDS KO clone 18)CVCL_HL95 (mitoLUHMES)
Sex of cell Female
Age at sampling 8FW
Category Conditionally immortalized cell line
STR profile Source(s): ATCC=CRL-2927

Markers:
AmelogeninX
CSF1PO13,14
D2S133817,24
D3S135817,18
D5S81811,13
D7S82011,13
D8S117912,13
D13S3179,11
D16S53911,12
D18S5112
D19S43314,15
D21S1130,31
FGA19,21
Penta D12,13
Penta E11,13
TH017,9.3
TPOX8
vWA14,17

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Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/growth-and-morphology-of-luhmes-crl-2927
Publications

PubMed=16000623; DOI=10.1523/JNEUROSCI.1746-05.2005; PMCID=PMC6725277
Julie Lotharius, Jeppe Falsig, Johan van Beek, Sarah Payne, Ralf Dringen, Patrik Brundin, Marcel Leist;
Progressive degeneration of human mesencephalic neuron-derived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed-lineage kinase pathway.
J. Neurosci. 25:6329-6342(2005)

PubMed=21434924; DOI=10.1111/j.1471-4159.2011.07255.x
Diana Scholz, Dominik Poltl, Andreas Genewsky, Matthias Weng, Tanja Waldmann, Stefan Schildknecht, Marcel Leist;
Rapid, complete and large-scale generation of post-mitotic neurons from the human LUHMES cell line.
J. Neurochem. 119:957-971(2011)

PubMed=24173167; DOI=10.14573/altex.2013.4.427
Stefan Schildknecht, Christiaan Karreman, Dominik Poltl, Liudmila Efremova, Cornelius Kullmann, Simon Gutbier, Anne K. Krug, Diana Scholz, Hanne R. Gerding, Marcel Leist;
Generation of genetically-modified human differentiated cells for toxicological tests and the study of neurodegenerative diseases.
ALTEX 30:427-444(2013)

PubMed=30602607; DOI=10.1128/JVI.02210-18; PMCID=PMC6401467
Terri G. Edwards, David C. Bloom;
Lund human mesencephalic (LUHMES) neuronal cell line supports herpes simplex virus 1 latency in vitro.
J. Virol. 93:e02210.18.1-e02210.18.14(2019)

PubMed=32150624; DOI=10.14573/altex.1911111
Markus Brull, Anna-Sophie Spreng, Simon Gutbier, Dominik Loser, Alice Krebs, Marvin Reich, Udo Kraushaar, Markus Britschgi, Christoph Patsch, Marcel Leist;
Incorporation of stem cell-derived astrocytes into neuronal organoids to allow neuro-glial interactions in toxicological studies.
ALTEX 37:409-428(2020)

Cross-references
Cell line collections (Providers) ABM; T0284
ATCC; CRL-2927
Biological sample resources BioSample; SAMN03471701
BioSample; SAMN08723614
Encyclopedic resources Wikidata; Q54903259
Entry history
Entry creation06-Jun-2012
Last entry update14-Aug-2025
Version number20