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Cellosaurus Rev-CEM-G2 (CVCL_A9NS)

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Cell line name Rev-CEM-G2
Synonyms RevCEM-G2; RevCEM clone G2
Accession CVCL_A9NS
Resource Identification Initiative To cite this cell line use: Rev-CEM-G2 (RRID:CVCL_A9NS)
Comments Population: Caucasian.
Characteristics: Transfected with a pNL-GFP-RRE(SA) construct that results in the expression of the GFP gene under the control of the HIV-1 LTR promoter in HIV-1 infected cells when Tat and Rev are present.
Characteristics: Expresses the mCherry gene under the control of a EF-1a promoter.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; HGNC:13222; BCL11B + HGNC; HGNC:2488; NKX2-5; Name(s)=NKX2.5-BCL11B (from parent cell line).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg465Cys (c.1393C>T); ClinVar=VCV000376414; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3765; FLT3; Simple; p.Ala627Thr (c.1879G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1593_Arg1594insProArgLeuProHisAsnSerSerPheHisPheLeu (c.4780_4781ins36); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Pro2412Thr (c.7234C>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A9NR (Rev-CEM-E7)
Sex of cell Female
Age at sampling 3Y11M
Category Cancer cell line
Publications

PubMed=27812216; DOI=10.1371/journal.ppat.1005964; PMCID=PMC5094736
Mikael Boulle, Thorsten G. Muller, Sabrina Dahling, Yashica Ganga, Laurelle Jackson, Deeqa Mahamed, Lance Oom, Gila Lustig, Richard A. Neher, Alex Sigal;
HIV cell-to-cell spread results in earlier onset of viral gene expression by multiple infections per cell.
PLoS Pathog. 12:e1005964.1-e1005964.23(2016)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-13436
Cell line databases/resources cancercelllines; CVCL_A9NS
Encyclopedic resources Wikidata; Q108821222
Entry history
Entry creation23-Sep-2021
Last entry update19-Dec-2024
Version number9