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Cellosaurus CHO-gmt4D (CVCL_A8SP)

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Cell line name CHO-gmt4D
Accession CVCL_A8SP
Resource Identification Initiative To cite this cell line use: CHO-gmt4D (RRID:CVCL_A8SP)
Comments Characteristics: Lacks N-acetylglucosaminyltransferase I activity.
Knockout cell: Method=ZFN; UniProtKB; Q2MH30; Dhfr.
Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; Q924C0; Mgat1; Simple; p.Gln339Ter (c.1015C>T); Zygosity=Unspecified (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_W354 (CHO-gmt4)
Children:
CVCL_A8SQ (CHO-gmt4D-EPO-GnT I)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

CLPUB00638
John Soo Yan Goh;
Applications of a novel CHO glycosylation mutant.
Thesis PhD (2014); National University of Singapore; Singapore; Singapore

PubMed=24166780; DOI=10.1002/biot.201300301
John Soo Yan Goh, Ying-Wei Liu, Hai-Feng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiang-Shan Zhou, Fu-Sheng Xie, Pei-Qing Zhang, Yuan-Xing Zhang, Zhi-Wei Song;
Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function.
Biotechnol. J. 9:100-109(2014)

Cross-references
Encyclopedic resources Wikidata; Q108820034
Entry history
Entry creation23-Sep-2021
Last entry update30-Jan-2024
Version number6