Cellosaurus cell line BRCi014-A-2 (CVCL

Cross-references
Cell line name	BRCi014-A-2
Synonyms	HPS5038; ISL1-TEZ_2
Accession	CVCL_A8KN
Resource Identification Initiative	To cite this cell line use: BRCi014-A-2 (RRID:CVCL_A8KN)
Comments	Population: Asian. Characteristics: Using CRISPR/Cas9 a 2A-tdTomato-EF1a-BleoR construct was knocked-in at the 3'end of one allele of the ISL1 gene (PubMed=34087992). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_UP39 (1383D6)
Sex of cell	Male
Age at sampling	36Y
Category	Induced pluripotent stem cell
Publications	PubMed=34087992; DOI=10.1016/j.scr.2021.102363 Satomi Tsukamoto, Koji Nakade, Tamami Wakabayashi, Kenichi Nakashima, Miho Takami, Yasuko Hemmi, Yuji Kuramochi, Tomoya Shimizu, Yutaka Arai, Mami Matsuo-Takasaki, Michiya Noguchi ...Show all 14 authors... , Yukio Nakamura, Yoshihiro Miwa, Yohei Hayashi; Show fewer authors Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53:102363-102363(2021)
Cell line databases/resources	hPSCreg; BRCi014-A-2
Encyclopedic resources	Wikidata; Q108819963
Entry history
Entry creation	23-Sep-2021
Last entry update	19-Dec-2024
Version number	7