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Cellosaurus K562/GM-CSF (CVCL_A8HT)

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Cell line name K562/GM-CSF
Synonyms K562 GM-CSF; K562GM-CSF; GM-K562
Accession CVCL_A8HT
Resource Identification Initiative To cite this cell line use: K562/GM-CSF (RRID:CVCL_A8HT)
Comments Population: Caucasian.
Biotechnology: Used for the production of the Sorento Therapeutics STI-6991 SARS-CoV-2 (COVID-19) vaccine candidate. The cell line which is lethally irradiated is used to produce the recombinant SARS-CoV-2 spike protein rSARS-CoV-2 which is the active part of the vaccine formulation (PubMed=32289117).
Characteristics: Does not express HLA class I or II antigens, potentially decreasing the magnitude of the alloresponses generated on repeated immunizations (PubMed=10466632).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:2434; CSF2.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0004 (K-562)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
Publications

PubMed=10466632; DOI=10.1089/10430349950017347
Ivan Borrello, Eduardo M. Sotomayor, Sara Cooke, Hyam I. Levitsky;
A universal granulocyte-macrophage colony-stimulating factor-producing bystander cell line for use in the formulation of autologous tumor cell-based vaccines.
Hum. Gene Ther. 10:1983-1991(1999)

PubMed=23170257; DOI=10.4161/onci.20954; PMCID=PMC3494623
Ana Brusic, Ursula Hainz, Martha Wadleigh, Donna Neuberg, Mei Su, Christine M. Canning, Daniel J. DeAngelo, Richard M. Stone, Jeng-Shin Lee, Richard C. Mulligan, Jerome Ritz ...Show all 14 authors... , Glenn Dranoff, Tetsuro Sasada, Catherine Ju-Ying Wu; Show fewer authors
Detecting T-cell reactivity to whole cell vaccines: proof of concept analysis of T-cell response to K562 cell antigens in CML patients.
OncoImmunology 1:1095-1103(2012)

PubMed=32289117; DOI=10.1016/j.medidd.2020.100026; PMCID=PMC7144842
Henry Ji, Ying Yan, Bei-Bei Ding, Wen-Zhong Guo, Mark Brunswick, Andreas G. Niethammer, Williams SooHoo, Robin Smith, Alexis Nahama, Yan-Liang Zhang;
Novel decoy cellular vaccine strategy utilizing transgenic antigen-expressing cells as immune presenter and adjuvant in vaccine prototype against SARS-CoV-2 virus.
Med. Drug Discov. 5:100026.1-100026.2(2020)

Cross-references
Encyclopedic resources Wikidata; Q107115522
Entry history
Entry creation20-May-2021
Last entry update19-Dec-2024
Version number9