Cellosaurus HEK-Blue-Lucia TNF-alpha (CVCL_A8CX)
| Cell line name | HEK-Blue-Lucia TNF-alpha |
|---|---|
| Synonyms | HEK-Dual TNF-alpha |
| Accession | CVCL_A8CX |
| Resource Identification Initiative | To cite this cell line use: HEK-Blue-Lucia TNF-alpha (RRID:CVCL_A8CX) |
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line both genes are under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites and 3 c-Rel binding sites (InvivoGen=hkd-tnfa). Knockout cell: Method=Not specified; HGNC; HGNC:7562; MYD88. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Cell line collections (Providers) | InvivoGen; hkd-tnfa |
| Encyclopedic resources | Wikidata; Q107115298 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 14-Aug-2025 |
| Version number | 9 |