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Cellosaurus RUCDRi002-A-17 (CVCL_A7LT)

[Text version]
Cell line name RUCDRi002-A-17
Synonyms TC-1133-CRISPRi2; UMGi147-A-17
Accession CVCL_A7LT
Resource Identification Initiative To cite this cell line use: RUCDRi002-A-17 (RRID:CVCL_A7LT)
Comments From: University Medical Center Goettingen; Goettingen; Germany.
Population: Hispanic.
Characteristics: Using CRISPR/Cas9 a CAG-dCas9-KRAB-T2A-tdTomato-WPRE construct was introduced in the AAVS1 safe harbor locus.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RH35 (ND50038)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Web pages https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=480
Publications

PubMed=34343828; DOI=10.1016/j.scr.2021.102473
Schoger E., Zimmermann W.-H., Cyganek L., Zelarayan L.C.
Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression.
Stem Cell Res. 55:102473-102473(2021)

Cross-references
Cell line databases/resources hPSCreg; RUCDRi002-A-17
Encyclopedic resources Wikidata; Q107116792
Entry history
Entry creation20-May-2021
Last entry update30-Jan-2024
Version number7