Cellosaurus logo
expasy logo
Due to maintenance work, this service will be unavailable on Monday between 06:00 until 06:30 CEST. Apologies for the inconvenience.

Cellosaurus RUCDRi002-A-15 (CVCL_A7LR)

[Text version]
Cell line name RUCDRi002-A-15
Synonyms TC-1133-CRISPRa2; hiPSC-CRISPRa2; UMGi147-A-15
Accession CVCL_A7LR
Resource Identification Initiative To cite this cell line use: RUCDRi002-A-15 (RRID:CVCL_A7LR)
Comments From: University Medical Center Goettingen; Goettingen; Germany.
Population: Hispanic.
Characteristics: Using CRISPR/Cas9 a dCas9-VPR-2A-tdTomato-WPRE construct containing dCas9 fused to heterotrimeric VPR transactivator consisting of VP64, p65, and RTA domains along with a tdTomato and a woodchuck hepatitis post-transcriptional regulatory element (WPRE) was introduced in the AAVS1 safe harbor locus (PubMed=34481190).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RH35 (ND50038)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Web pages https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=478

PubMed=34481190; DOI=10.1016/j.scr.2021.102518
Schoger E., Zimmermann W.-H., Cyganek L., Zelarayan L.C.
Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation.
Stem Cell Res. 56:102518-102518(2021)

Cell line databases/resources hPSCreg; RUCDRi002-A-15
Encyclopedic resources Wikidata; Q107116790
Entry history
Entry creation20-May-2021
Last entry update30-Jan-2024
Version number7