Cellosaurus cell line HEK-DsupKD (CVCL

Cellosaurus HEK-DsupKD (CVCL_A7AX)

Cross-references
Cell line name	HEK-DsupKD
Accession	CVCL_A7AX
Resource Identification Initiative	To cite this cell line use: HEK-DsupKD (RRID:CVCL_A7AX)
Comments	Characteristics: Expresses Dsup under the control of a constitutive CAG promoter (PubMed=27649274). Knockout cell: Method=shRNA knockdown; UniProtKB; P0DOW4; Ramazzottius varieornatus Dsup. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P0DOW4; Ramazzottius varieornatus Dsup. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_A7AW (HEK-Dsup)
Sex of cell	Female
Age at sampling	Fetus
Category	Transformed cell line
Publications	PubMed=27649274; DOI=10.1038/ncomms12808; PMCID=PMC5034306 Takuma Hashimoto, Daiki D. Horikawa, Yuki Saito, Hirokazu Kuwahara, Hiroko Kozuka-Hata, Tadasu Shin-I, Yohei Minakuchi, Kazuko Ohishi, Ayuko Motoyama, Tomoyuki Aizu, Atsushi Enomoto ...Show all 28 authors... , Koyuki Kondo, Sae Tanaka, Yuichiro Hara, Shigeyuki Koshikawa, Hiroshi Sagara, Toru Miura, Shin-ichi Yokobori, Kiyoshi Miyagawa, Yutaka Suzuki, Takeo Kubo, Masaaki Oyama, Yuji Kohara, Asao Fujiyama, Kazuharu Arakawa, Toshiaki Katayama, Atsushi Toyoda, Takekazu Kunieda; Show fewer authors Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nat. Commun. 7:12808.1-12808.14(2016)
Cell line collections (Providers)	RCB; RCB4688
Encyclopedic resources	Wikidata; Q107115288
Entry history
Entry creation	20-May-2021
Last entry update	14-Aug-2025
Version number	7