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Cellosaurus FM3A tsFT5 (CVCL_A5JX)

[Text version]
Cell line name FM3A tsFT5
Synonyms tsFT5
Accession CVCL_A5JX
Resource Identification Initiative To cite this cell line use: FM3A tsFT5 (RRID:CVCL_A5JX)
Comments Characteristics: Temperature sensitive phenotype. Stop growing at 39 Celsius. Defective in DNA replication. Contains a thermolabile Uba1 (PubMed=8344958).
Doubling time: ~18 hours (PubMed=8344958).
Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Disease Malignant neoplasms of the mouse mammary gland (NCIt: C21678)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H/He.
Hierarchy Parent: CVCL_3869 (FM3A)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
Publications

PubMed=8344958; DOI=10.1016/S0021-9258(19)85487-X
Masahiro Mori, Toshihiko Eki, Mami Takahashi-Kudo, Fumio Hanaoka, Michio Ui, Takemi Enomoto;
Characterization of DNA synthesis at a restrictive temperature in the temperature-sensitive mutants, tsFT5 cells, that belong to the complementation group of ts85 cells containing a thermolabile ubiquitin-activating enzyme E1. Involvement of the ubiquitin-conjugating system in DNA replication.
J. Biol. Chem. 268:16803-16809(1993)

Cross-references
Encyclopedic resources Wikidata; Q107115052
Entry history
Entry creation20-May-2021
Last entry update14-Aug-2025
Version number6