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Cellosaurus HEK293T-DDX6-null (CVCL_A5EF)

[Text version]
Cell line name HEK293T-DDX6-null
Synonyms HEK293T Ddx6-null
Accession CVCL_A5EF
Resource Identification Initiative To cite this cell line use: HEK293T-DDX6-null (RRID:CVCL_A5EF)
Comments Doubling time: ~30-40 hours (DSMZ=ACC-873).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:2747; DDX6.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): DSMZ=ACC-873

Markers:
AmelogeninX
CSF1PO11,12
D2S133818,19
D3S135815,16,17
D5S8188,9
D7S82010,11
D8S117912,14
D13S31711,12,14
D16S5399,15
D18S5117,18
D19S43318,19
D21S1128,30.2
FGA23
Penta D9,10
Penta E7,15
TH017,9.3
TPOX10,11
vWA16,19,20

Run an STR similarity search on this cell line
Publications

PubMed=31570513; DOI=10.26508/lsa.201900405; PMCID=PMC6769256
Aoife Hanet, Felix Rasch, Ramona Weber, Vincenzo Ruscica, Maria Fauser, Tobias Raisch, Duygu Kuzuoglu-Ozturk, Chung-Te Chang, Dipankar Bhandari, Catia Igreja, Lara Wohlbold;
HELZ directly interacts with CCR4-NOT and causes decay of bound mRNAs.
Life Sci. Alliance 2:e201900405.1-e201900405.16(2019)

Cross-references
Cell line collections (Providers) DSMZ; ACC-873
Cell line databases/resources DSMZCellDive; ACC-873
Encyclopedic resources Wikidata; Q107115307
Entry history
Entry creation20-May-2021
Last entry update14-Aug-2025
Version number10