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Cellosaurus WMUi013-A (CVCL_A4EM)

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Cell line name WMUi013-A
Synonyms iPS-Cas9; WMUi006-A
Accession CVCL_A4EM
Resource Identification Initiative To cite this cell line use: WMUi013-A (RRID:CVCL_A4EM)
Comments From: Wenzhou Medical University; Wenzhou; China.
Population: Chinese; Han.
Characteristics: Using CRISPR/Cas9 a HA/L-TetOn-Cas9-CMV-PuroR-2A-HA/R construct was introduced in the AAVS1 safe harbor locus (PubMed=33207306).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from site: In situ; Urine; UBERON=UBERON_0001088.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 37Y
Category Induced pluripotent stem cell
Publications

PubMed=33207306; DOI=10.1016/j.scr.2020.102064
Yin-Juan Ding, Long Wang, Wei-Ping Ji, Zhan-Guo Chen, De-Xuan Wang, Cong-De Chen, Hong-Fei Tong, Zhao Han, Chao Niu, Mao-Ping Chu, Jin-Yu Huang, Xiao-Ling Guo;
Generation of a human induced pluripotent stem cell line with Cas9 driven by Tet-on operator via AAVS1 safe harbor gene-editing.
Stem Cell Res. 49:102064-102064(2020)

Cross-references
Cell line databases/resources hPSCreg; WMUi013-A
Encyclopedic resources Wikidata; Q105511759
Entry history
Entry creation12-Jan-2021
Last entry update14-Aug-2025
Version number8