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Cellosaurus RUCDRi002-A-5 (CVCL_A1PK)

[Text version]
Cell line name RUCDRi002-A-5
Synonyms TC-1133-CRISPRa; UMGi147-A-5
Accession CVCL_A1PK
Resource Identification Initiative To cite this cell line use: RUCDRi002-A-5 (RRID:CVCL_A1PK)
Comments From: University Medical Center Goettingen; Goettingen; Germany.
Population: Hispanic.
Characteristics: Using CRISPR/Cas9 two constructs one containing CAGpromoter-dCas9-VPR-2A-tdTomato-gGH and the other EF1a-promoter-Pac-SV40polyA were introduced in the AAVS1 safe harbor locus (PubMed=33038615).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_RH35 (ND50038)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Web pages https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=390

PubMed=33038615; DOI=10.1016/j.scr.2020.101944
Schoger E., Argyriou L., Zimmermann W.-H., Cyganek L., Zelarayan L.C.
Generation of homozygous CRISPRa human induced pluripotent stem cell (hiPSC) lines for sustained endogenous gene activation.
Stem Cell Res. 48:101944-101944(2020)

Cell line databases/resources hPSCreg; RUCDRi002-A-5
Encyclopedic resources Wikidata; Q102114853
Entry history
Entry creation29-Oct-2020
Last entry update30-Jan-2024
Version number9