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Cellosaurus SEES3-1V human ESRRB, clone2 (CVCL_A1L4)

[Text version]
Cell line name SEES3-1V human ESRRB, clone2
Synonyms 1V-90930-3B
Accession CVCL_A1L4
Resource Identification Initiative To cite this cell line use: SEES3-1V human ESRRB, clone2 (RRID:CVCL_A1L4)
Comments Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed.
Characteristics: Transposed with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein.
Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:3473; ESRRB.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_ZJ91 (SEES3-1V)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=32433964; DOI=10.1016/j.celrep.2020.107655
Yuhki Nakatake, Shigeru B.H. Ko, Alexei A. Sharov, Shunichi Wakabayashi, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Chiaki Ookura, Saeko Sato, Noriko Ito, Madoka Ishikawa-Hirayama ...Show all 27 authors... , Siu Shan Mak, Lars Martin Jakt, Tomoo Ueno, Ken Hiratsuka, Misako Matsushita, Sravan K. Goparaju, Tomohiko Akiyama, Kei-ichiro Ishiguro, Mayumi Oda, Norio Gouda, Akihiro Umezawa, Hidenori Akutsu, Kunihiro Nishimura, Ryo Matoba, Osamu Ohara, Minoru S.H. Ko; Show fewer authors
Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors.
Cell Rep. 31:107655.1-107655.23(2020)

Cross-references
Cell line collections (Providers) RCB; HES0012
Encyclopedic resources Wikidata; Q98130239
Entry history
Entry creation02-Jul-2020
Last entry update14-Aug-2025
Version number11