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Cellosaurus SEES3-1V human EOMES, clone1 (CVCL_A1J8)

[Text version]
Cell line name SEES3-1V human EOMES, clone1
Synonyms 1V-91688-4A
Accession CVCL_A1J8
Resource Identification Initiative To cite this cell line use: SEES3-1V human EOMES, clone1 (RRID:CVCL_A1J8)
Comments Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed.
Characteristics: Transposed with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein.
Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:3372; EOMES.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_ZJ91 (SEES3-1V)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=32433964; DOI=10.1016/j.celrep.2020.107655
Nakatake Y., Ko S.B.H., Sharov A.A., Wakabayashi S., Murakami M., Sakota M., Chikazawa-Nohtomi N., Ookura C., Sato S., Ito N., Ishikawa-Hirayama M., Mak S.S., Jakt L.M., Ueno T., Hiratsuka K., Matsushita M., Goparaju S.K., Akiyama T., Ishiguro K.-i., Oda M., Gouda N., Umezawa A., Akutsu H., Nishimura K., Matoba R., Ohara O., Ko M.S.H.
Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors.
Cell Rep. 31:107655.1-107655.23(2020)

Cross-references
Cell line collections (Providers) RCB; HES0013
Encyclopedic resources Wikidata; Q98130211
Entry history
Entry creation02-Jul-2020
Last entry update10-Apr-2025
Version number10