Cellosaurus SEES3-1V human EOMES, clone1 (CVCL_A1J8)
| Cell line name | SEES3-1V human EOMES, clone1 |
|---|---|
| Synonyms | 1V-91688-4A |
| Accession | CVCL_A1J8 |
| Resource Identification Initiative | To cite this cell line use: SEES3-1V human EOMES, clone1 (RRID:CVCL_A1J8) |
| Comments | Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed. Characteristics: Transfected with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a PiggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein. Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Transfected with: HGNC; 3372; EOMES. Transfected with: UniProtKB; P00722; Escherichia coli lacZ. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_ZJ91 (SEES3-1V) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=32433964; DOI=10.1016/j.celrep.2020.107655 |
| Cross-references | |
| Cell line collections | RCB; HES0013 |
| Encyclopedic resources | Wikidata; Q98130211 |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 21-Mar-2023 |
| Version number | 5 |