Cellosaurus SEES3-1V human EOMES, clone1 (CVCL_A1J8)
Cell line name | SEES3-1V human EOMES, clone1 |
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Synonyms | 1V-91688-4A |
Accession | CVCL_A1J8 |
Resource Identification Initiative | To cite this cell line use: SEES3-1V human EOMES, clone1 (RRID:CVCL_A1J8) |
Comments | Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed. Characteristics: Transposed with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein. Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Genetic integration: Method=PiggyBac transposition; Gene=HGNC; 3372; EOMES. Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ). Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection; Gene=UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_ZJ91 (SEES3-1V) |
Sex of cell | Male |
Age at sampling | Blastocyst stage |
Category | Embryonic stem cell |
Publications | PubMed=32433964; DOI=10.1016/j.celrep.2020.107655 |
Cross-references | |
Cell line collections (Providers) | RCB; HES0013 |
Encyclopedic resources | Wikidata; Q98130211 |
Entry history | |
Entry creation | 02-Jul-2020 |
Last entry update | 10-Sep-2024 |
Version number | 8 |