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Cellosaurus SNUe003-A-3 (CVCL_A0MK)

[Text version]
Cell line name SNUe003-A-3
Synonyms SNUhES3-alphaMHC-EGFP; A-eG-KI-SE-3; IRMPe004-A-3
Accession CVCL_A0MK
Resource Identification Initiative To cite this cell line use: SNUe003-A-3 (RRID:CVCL_A0MK)
Comments From: Seoul National University; Seoul; South Korea.
Population: Korean.
Characteristics: Using CRISPR/Cas9 a T2A-EGFP-polyA-PGKp-PuroR-polyA construct was introduced at the C-terminus of MYH6 (PubMed=35427904).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9949 (SNUhES3)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=35427904; DOI=10.1016/j.scr.2022.102779
Ha Myoung Lee, A-Hyeon Kim, Sunsook Hwang, Juwon Jung, Hyewon Seol, Jin Jea Sung, Seung Min Jeong, Young Min Choi, Jong Kwan Jun, Han-Soo Kim, Jiho Jang;
Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting.
Stem Cell Res. 61:102779-102779(2022)

Cross-references
Cell line databases/resources hPSCreg; SNUe003-A-3
Biological sample resources BioSamples; SAMEA9403864
Encyclopedic resources Wikidata; Q108821319
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number9