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Cellosaurus CHLA-258 (CVCL_A058)

[Text version]
Cell line name CHLA-258
Synonyms CHLA258
Accession CVCL_A058
Resource Identification Initiative To cite this cell line use: CHLA-258 (RRID:CVCL_A058)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: MD Anderson Cell Lines Project.
Part of: NCI Pediatric Preclinical Testing Program (PPTP) cell line panel.
Doubling time: 71 hours (PubMed=20922763); 89 hours (PubMed=24312454).
Omics: Deep exome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (PubMed=25010205).
  • Gene fusion; HGNC; 3508; EWSR1 + HGNC; 3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 3 fusion (Ex10/Ex6) (PubMed=25010205).
Disease Primitive neuroectodermal tumor (NCIt: C3716)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): COG; PubMed=20922763; PubMed=24312454; PubMed=25010205

Markers:
AmelogeninX
CSF1PO10,11
D2S133817,23
D3S135815
D5S8187,11
D7S82011,12
D8S117914,15
D13S3179,11
D16S53910 (PubMed=20922763; PubMed=25010205)
10,12 (COG; PubMed=24312454)
D18S5112,17
D19S43314
D21S1131.2
FGA19,25
TH017,9.3
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://www.cccells.org/PDF_Files/Brain/CHLA-258%20Cell%20Line%20Data%20Sheet.pdf
https://www.cccells.org/tables/Ewings.php
http://www.pptpinvitro.org/cell_lines_panel.php
https://tcpaportal.org/mclp/
Publications

PubMed=15289350; DOI=10.1158/0008-5472.CAN-04-0377
Batra S., Reynolds C.P., Maurer B.J.
Fenretinide cytotoxicity for Ewing's sarcoma and primitive neuroectodermal tumor cell lines is decreased by hypoxia and synergistically enhanced by ceramide modulators.
Cancer Res. 64:5415-5424(2004)

PubMed=20922763; DOI=10.1002/pbc.22801
Kang M.H., Smith M.A., Morton C.L., Keshelava N., Houghton P.J., Reynolds C.P.
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
Pediatr. Blood Cancer 56:239-249(2011)

PubMed=24312454; DOI=10.1371/journal.pone.0080060
May W.A., Grigoryan R.S., Keshelava N., Cabral D.J., Christensen L.L., Jenabi J., Ji L.-Y., Triche T.J., Lawlor E.R., Reynolds C.P.
Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.
PLoS ONE 8:E80060-E80060(2013)

PubMed=25010205; DOI=10.1371/journal.pgen.1004475
Brohl A.S., Solomon D.A., Chang W., Wang J.-J., Song Y., Sindiri S., Patidar R., Hurd L., Chen L., Shern J.F., Liao H.-L., Wen X.-Y., Gerard J., Kim J.-S., Lopez Guerrero J.A., Machado I., Wai D.H., Picci P., Triche T.J., Horvai A.E., Miettinen M.M., Wei J.S., Catchpoole D., Llombart-Bosch A., Waldman T., Khan J.
The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:E1004475-E1004475(2014)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074
Teicher B.A., Polley E.C., Kunkel M., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line databases/resources cancercelllines; CVCL_A058
Cell_Model_Passport; SIDM01720
DepMap; ACH-001282
Encyclopedic resources Wikidata; Q54812494
Gene expression databases GEO; GSM1676354
GEO; GSM1701684
Polymorphism and mutation databases Cosmic; 1112131
Cosmic; 2228217
Cosmic; 2294582
Entry history
Entry creation06-Jun-2012
Last entry update05-Oct-2023
Version number21