Cellosaurus cell line HCjE (CVCL

Cellosaurus HCjE (CVCL_9700)

[Text version]

Cell line name

HCjE

Synonyms

HCjE-Gi; ConjEp-1/p53DD/cdk4R/TERT

Accession

CVCL_9700

Resource Identification Initiative

To cite this cell line use: HCjE (RRID:CVCL_9700)

Comments

Characteristics: Can form 3D spheroids (PubMed=32545782).
Karyotypic information: Has lost chromosome Y (PubMed=29787296).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1773; CDK4 (Note=With p.Arg24Cys).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=MGI; MGI:98834; Trp53 (Note=With p.Leu14_Lys302del = p53DD).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Eye, conjunctival epithelium; UBERON=UBERON_0006763.
Cell type: Keratinocyte; CL=CL_0000312.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_UZ27 (ConjEp-1)

Sex of cell

Male

Age at sampling

82Y

Category

Telomerase immortalized cell line

STR profile

Source(s): PubMed=29787296

Markers:

Amelogenin	X
CSF1PO	11
D3S1358	16,18
D5S818	11,13
D7S820	10,12
D8S1179	11,12
D13S317	11,12
D16S539	12
D18S51	15
D21S11	31,34.2
FGA	20,24
Penta D	9,10
Penta E	12
TH01	9
TPOX	8,11
vWA	14

Web pages

Provider; BWH dermatology; -; https://dermatology.bwh.harvard.edu/docs/Cell-Culture-Core-Cell-Lines.pdf

Publications

PubMed=12077343; DOI=10.1128/mcb.22.14.5157-5172.2002; PMCID=PMC139780
James George Rheinwald, William Chun Hahn, Matthew Robert Ramsey, Jenny Y. Wu, Zong-You Guo, Hensin Tsao, Michele De Luca, Caterina Catricala, Kathleen M. O'Toole;
A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status.
Mol. Cell. Biol. 22:5157-5172(2002)

PubMed=12766048; DOI=10.1167/iovs.02-0851
Ilene K. Gipson, Sandra Spurr-Michaud, Pablo Argueso, Ann Tisdale, Tat Fong Ng, Cindy Leigh Russo;
Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines.
Invest. Ophthalmol. Vis. Sci. 44:2496-2506(2003)

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
Alison M. McDermott, Hasna Baidouri, Ashley M. Woodward, Wendy R. Kam, Yang Liu, Xiao-Min Chen, Jillian F. Ziemanski, Kerry Vistisen, Linda D. Hazlett, Kelly K. Nichols, Pablo Argueso, David A. Sullivan;
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

PubMed=32545782; DOI=10.3390/biomedicines8060158; PMCID=PMC7344416
Miltiadis Fiorentzis, Periklis Katopodis, Helen Kalirai, Berthold Seitz, Arne Viestenz, Sarah E. Coupland;
Image analysis of 3D conjunctival melanoma cell cultures following electrochemotherapy.
Biomedicines 8:158.1-158.13(2020)

Cross-references

Encyclopedic resources

Wikidata; Q54881879

Experimental variables resources

EFO; EFO_0003139

Entry history

Entry creation

06-Jun-2012

Last entry update

14-Aug-2025

Version number