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Cellosaurus KM12-C (CVCL_9547)

[Text version]
Cell line name KM12-C
Synonyms KM12 C; KM-12C; KM12C; KM12c; Km12C
Accession CVCL_9547
Resource Identification Initiative To cite this cell line use: KM12-C (RRID:CVCL_9547)
Comments Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12533.
Registration: Chiron Master Culture Collection; CMCC 11611 (CMCC #11611).
Population: Caucasian.
Microsatellite instability: Instable (MSI) (PubMed=25926053).
Omics: Proteomics; Subcellular; Nucleus.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene fusion; HGNC; HGNC:8031; NTRK1 + HGNC; HGNC:12012; TPM3; Name(s)=TPM3-NTRK1 (from parent cell line).
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Asn1819fs*7 (c.5454_5455insA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Asn1784Hisfs*7 (c.5350_5351delAA); ClinVar=VCV000037959; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Gly129Ter (c.385G>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA); ClinVar=VCV000092828; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val73Trpfs*50 (c.216delC) (p.P72fs); ClinVar=VCV000428897; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Arg (c.536A>G); ClinVar=VCV000376606; Zygosity=Heterozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1331 (KM12)
Children:
CVCL_4M93 (KM12-HX)CVCL_4M94 (KM12-LX)CVCL_9548 (KM12-SM)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): PubMed=25926053

Markers:
AmelogeninX
CSF1PO10
D5S81810
D7S8208,9
D13S31712,15
D16S53911
D21S1127,34.2
TH0110
TPOX11,12
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=2846163
Kiyoshi Morikawa, Shirley M. Walker, Motowo Nakajima, Sen Pathak, John Milburn Jessup, Isaiah Joshua Fidler;
Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice.
Cancer Res. 48:6863-6871(1988)

PubMed=3349467
Kiyoshi Morikawa, Shirley M. Walker, John Milburn Jessup, Isaiah Joshua Fidler;
In vivo selection of highly metastatic cells from surgical specimens of different primary human colon carcinomas implanted into nude mice.
Cancer Res. 48:1943-1948(1988)

PubMed=10091941; DOI=10.1023/A:1006537609469
Yoshifumi Nakayama, Keisuke Okazaki, Kazunori Shibao, Tatsuhiko Sako, Keiji Hirata, Naoki Nagata, Michihiko Kuwano, Hideaki Itoh;
Alterative expression of the collagenase and adhesion molecules in the highly metastatic clones of human colonic cancer cell lines.
Clin. Exp. Metastasis 16:461-469(1998)

PubMed=15170669; DOI=10.1002/ijc.20195
Jordi Camps, Cristina Morales, Esther Prat, Maria Ribas, Gabriel Capella, Josep Egozcue, Miguel Angel Peinado, Rosa Miro;
Genetic evolution in colon cancer KM12 cells and metastatic derivates.
Int. J. Cancer 110:869-874(2004)

PubMed=25926053; DOI=10.1038/ncomms8002
Enzo Medico, Mariangela Russo, Gabriele Picco, Carlotta Cancelliere, Emanuele Valtorta, Giorgio Corti, Michela Buscarino, Claudio Isella, Simona Lamba, Barbara Martinoglio, Silvio Veronese ...Show all 19 authors... , Salvatore Siena, Andrea Sartore-Bianchi, Marco Beccuti, Marcella Mottolese, Michael Linnebacher, Francesca Cordero, Federica Di Nicolantonio, Alberto Bardelli; Show fewer authors
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=29131639; DOI=10.1021/acs.jproteome.7b00548
Sofia Torres, Irene Garcia-Palmero, Consuelo Marin-Vicente, Ruben A. Bartolome, Eva Calvino, Maria Jesus Fernandez-Acenero, J. Ignacio Casal;
Proteomic characterization of transcription and splicing factors associated with a metastatic phenotype in colorectal cancer.
J. Proteome Res. 17:252-264(2018)

Cross-references
Cell line collections (Providers) KCLB; 80015
Cell line databases/resources cancercelllines; CVCL_9547
Anatomy/cell type resources BTO; BTO_0003312
Chemistry resources ChEMBL-Cells; CHEMBL4632322
ChEMBL-Targets; CHEMBL4632329
PubChem_Cell_line; CVCL_9547
Encyclopedic resources Wikidata; Q54900054
Experimental variables resources EFO; EFO_0022404
Gene expression databases GEO; GSM1448186
GEO; GSM2299786
Polymorphism and mutation databases Cosmic; 2820474
Proteomic databases PRIDE; PXD006662
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number27