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Cellosaurus HS-Sch-2 (CVCL_8718)

[Text version]
Cell line name HS-Sch-2
Synonyms HS-sch-2; HSSCH2
Accession CVCL_8718
Resource Identification Initiative To cite this cell line use: HS-Sch-2 (RRID:CVCL_8718)
Comments Problematic cell line: Misclassified. Originally thought to be a malignant peripheral nerve sheath tumor (MPNST) but shown to be from a desmplastic melanoma (PubMed=36818284). This is based on a number of criteria such as the result of a methylome-based classifier, the expression of the SOX10 and S100B markers, a WT status for PRC2 genes, the complete inactivation of NF1 and CDKN2A and negative staining for Melan-A.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Japanese.
Doubling time: ~56 hours (PubMed=10891546).
Karyotypic information: Average ploidy of 3.89 (PubMed=36818284).
Omics: Genomics; Whole exome sequencing.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Peripheral nervous system, left sciatic nerve; UBERON=UBERON_0001322.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Heterozygous; Note=By translocation (PubMed=34059954).
  • Mutation; HGNC; HGNC:7765; NF1; Simple; p.Glu91Asnfs*6 (c.270_288del); Zygosity=Heterozygous; Note=Somatic (PubMed=36818284).
  • Mutation; HGNC; HGNC:7765; NF1; Simple; c.3113+1G>A; ClinVar=VCV000000345; Zygosity=Heterozygous; Note=Splice donor mutation. Somatic (PubMed=36818284).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (PubMed=10891546; DepMap=ACH-002066).
Disease Desmoplastic melanoma (NCIt: C37257)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): PubMed=28556483; PubMed=33707600; PubMed=36818284; RCB=RCB2230

Markers:
AmelogeninX
CSF1PO11,13
D2S133820
D3S135818
D5S81813
D7S8208,11 (PubMed=28556483; PubMed=36818284; RCB=RCB2230)
11 (PubMed=33707600)
D8S117914
D13S31711
D16S53911,12
D18S5113 (PubMed=33707600)
13,14 (PubMed=28556483; PubMed=36818284)
D19S43310.2,14.2 (PubMed=33707600)
14.2 (PubMed=28556483; PubMed=36818284)
D21S1129,30
FGA23,26
TH016,7
TPOX8
vWA15,16

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Publications

PubMed=10891546; DOI=10.3892/ijo.17.2.347
Sonobe H., Takeuchi T., Furihata M., Taguchi T., Kawai A., Ohjimi Y., Iwasaki H., Kaneko Y., Ohtsuki Y.
A new human malignant peripheral nerve sheath tumour-cell line, HS-Sch-2, harbouring p53 point mutation.
Int. J. Oncol. 17:347-352(2000)

PubMed=28556483; DOI=10.1002/1878-0261.12086; PMCID=PMC5579334
Kolberg M., Bruun J., Murumagi A., Mpindi J.P., Bergsland C.H., Holand M., Eilertsen I.A., Danielsen S.A., Kallioniemi O.-P., Lothe R.A.
Drug sensitivity and resistance testing identifies PLK1 inhibitors and gemcitabine as potent drugs for malignant peripheral nerve sheath tumors.
Mol. Oncol. 11:1156-1171(2017)

PubMed=32642732; DOI=10.1093/noajnl/vdz049; PMCID=PMC7317054
Pemov A., Li H., Presley W., Wallace M.R., Miller D.T.
Genetics of human malignant peripheral nerve sheath tumors.
Neurooncol. Adv. 2:i50-i61(2020)

PubMed=33707600; DOI=10.1038/s41598-021-85055-2; PMCID=PMC7952412
Longo J.F., Brosius S.N., Znoyko I., Alers V.A., Jenkins D.P., Wilson R.C., Carroll A.J. 3rd, Wolff D.J., Roth K.A., Carroll S.L.
Establishment and genomic characterization of a sporadic malignant peripheral nerve sheath tumor cell line.
Sci. Rep. 11:5690.1-5690.19(2021)

PubMed=34059954; DOI=10.1007/s00439-021-02296-x
Magallon-Lorenz M., Fernandez-Rodriguez J., Terribas E., Creus-Bachiller E., Romagosa C., Estival A., Perez Sidelnikova D., Salvador H., Villanueva A., Blanco I., Carrio M., Lazaro C., Serra E., Gel B.
Chromosomal translocations inactivating CDKN2A support a single path for malignant peripheral nerve sheath tumor initiation.
Hum. Genet. 140:1241-1252(2021)

PubMed=36818284; DOI=10.1016/j.isci.2023.106096; PMCID=PMC9929861
Magallon-Lorenz M., Terribas E., Ortega-Bertran S., Creus-Bachiller E., Fernandez M., Requena G., Rosas I., Mazuelas H., Uriarte-Arrazola I., Negro A., Lausova T., Castellanos E., Blanco I., DeVries G.H., Kawashima H., Legius E., Brems H., Mautner V.-F., Kluwe L., Ratner N., Wallace M.R., Fernandez-Rodriguez J., Lazaro C., Fletcher J.A., Reuss D.E., Carrio M., Gel B., Serra E.
Deep genomic analysis of malignant peripheral nerve sheath tumor cell lines challenges current malignant peripheral nerve sheath tumor diagnosis.
iScience 26:106096.1-106096.22(2023)

Cross-references
Cell line collections (Providers) BCRJ; 0376
RCB; RCB2230
Cell line databases/resources CLO; CLO_0050146
cancercelllines; CVCL_8718
DepMap; ACH-002066
Biological sample resources BioSample; SAMN03472028
Encyclopedic resources Wikidata; Q54896291
Polymorphism and mutation databases IARC_TP53; 17934
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number27