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Cellosaurus NS-3 (CVCL_8377)

[Text version]
Cell line name NS-3
Accession CVCL_8377
Resource Identification Initiative To cite this cell line use: NS-3 (RRID:CVCL_8377)
Comments Problematic cell line: Contaminated. Shown to be a COLO 201 derivative (PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00336.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Asn287Ser (c.860A>G); ClinVar=VCV000133954; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Derived from metastatic site: Ascites.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1987 (COLO 201)
Sex of cell Male
Age at sampling 70Y
Category Cancer cell line
STR profile Source(s): JCRB

Markers:
AmelogeninX
CSF1PO11,12
D5S81810,13
D7S8209,10
D13S31710,12
D16S53912,13
TH018,9
TPOX11
vWA15

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line collections JCRB; JCRB1076 - Discontinued
JCRB; NIHS0335 - Discontinued
Biological sample resources BioSample; SAMN03151625
Encyclopedic resources Wikidata; Q54931043
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number23