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Cellosaurus TC-32 (CVCL_7151)

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Cell line name TC-32
Synonyms TC32
Accession CVCL_7151
Resource Identification Initiative To cite this cell line use: TC-32 (RRID:CVCL_7151)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: Ewing Sarcoma Cell Line Atlas (ESCLA).
Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Doubling time: 24 hours (PubMed=24312454); 36 hours (PubMed=25984343).
Omics: Genomics; ChIP-seq; FLI1.
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Bone, left ilium; UBERON=UBERON_0001273.
Sequence variations
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.13
Native American0.3
East Asian, North1.12
East Asian, South0
South Asian0.2
European, North67.6
European, South30.65
Disease Primitive neuroectodermal tumor (NCIt: C3716)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 17Y
Category Cancer cell line
STR profile Source(s): PubMed=24312454; PubMed=25010205; PubMed=30879952

Markers:
AmelogeninX
CSF1PO11,13
D2S133817,19
D3S135815,16
D5S81812,13
D7S8208,11
D8S117912
D13S31710,12
D16S53913,14
D18S5111,19
D19S43312,14
D21S1127,28
FGA23,24
TH016,9.3
TPOX9,11
vWA15,18

Run an STR similarity search on this cell line
Web pages Info; ESCLA; -; https://hgserver1.amc.nl/cgi-bin/r2/main.cgi?option=about_dscope
Info; MCLP; -; https://tcpaportal.org/mclp/
Provider; CCR; -; https://www.cccells.org/PDF_Files/Ewings/TC-32.pdf
Provider; CCR; -; https://www.cccells.org/tables/Ewings.php
Publications

PubMed=3004699; DOI=10.1016/0165-4608(86)90001-4
Jacqueline Whang-Peng, Timothy J. Triche, Turid Knutsen, James S. Miser, Chien-Song Kao-Shan, Schickwann Tsai, Mark A. Israel;
Cytogenetic characterization of selected small round cell tumors of childhood.
Cancer Genet. Cytogenet. 21:185-208(1986)

PubMed=3390826
Catherine McKeon, Carol Jeanne Thiele, Robert A. Ross, Michael Kwan, Timothy J. Triche, James S. Miser, Mark A. Israel;
Indistinguishable patterns of protooncogene expression in two distinct but closely related tumors: Ewing's sarcoma and neuroepithelioma.
Cancer Res. 48:4307-4311(1988)

DOI=10.1016/B978-0-12-333530-2.50006-X
Mark A. Israel, Carol Jeanne Thiele;
Tumor cell lines of the peripheral nervous system.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.43-78; Academic Press; New York; USA (1994)

PubMed=8040301; DOI=10.1172/JCI117360; PMCID=PMC295111
Marco Giovannini, Jaclyn A. Biegel, Massimo Serra, Jian-Ying Wang, Ya-Lin H. Wei, Lynn M. Nycum, Beverly S. Emanuel, Glen A. Evans;
EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and primitive neuroectodermal tumors with variant translocations.
J. Clin. Invest. 94:489-496(1994)

PubMed=16631476; DOI=10.1016/j.cancergencyto.2005.11.006
Karoly Szuhai, Marije IJszenga, Hans J. Tanke, Carla Rosenberg, Pancras Cornelis Wilhelmus Hogendoorn;
Molecular cytogenetic characterization of four previously established and two newly established Ewing sarcoma cell lines.
Cancer Genet. Cytogenet. 166:173-179(2006)

PubMed=22142829; DOI=10.1158/1078-0432.CCR-11-2056; PMCID=PMC3271129
Neerav Shukla, Nabahet Ameur, Ismail Yilmaz, Khedoudja Nafa, Chyau-Yueh Lau, Angela Marchetti, Laetitia Borsu, Frederic G. Barr, Marc Ladanyi;
Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.
Clin. Cancer Res. 18:748-757(2012)

PubMed=24312454; DOI=10.1371/journal.pone.0080060; PMCID=PMC3846563
William A. May, Rita S. Grigoryan, Nino Keshelava, Daniel J. Cabral, Laura L. Christensen, Jasmine Jenabi, Ling-Yun Ji, Timothy J. Triche, Elizabeth R. Lawlor, Charles Patrick Reynolds;
Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.
PLoS ONE 8:e80060.1-e80060.10(2013)

PubMed=25010205; DOI=10.1371/journal.pgen.1004475; PMCID=PMC4091782
Andrew S. Brohl, David A. Solomon, Wendy Chang, Jian-Jun Wang, Young Song, Sivasish Sindiri, Rajesh Patidar, Laura Hurd, Li Chen, Jack F. Shern, Hong-Ling Liao ...Show all 26 authors... , Xin-Yu Wen, Julia Gerard, Jung-Sik Kim, Jose Antonio Lopez Guerrero, Isidro Machado, Daniel H. Wai, Piero Picci, Timothy J. Triche, Andrew E. Horvai, Markku Martti Miettinen, Jun S. Wei, Daniel Catchpoole, Antonio Llombart-Bosch, Todd A. Waldman, Javed Khan; Show fewer authors
The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:e1004475.1-e1004475.13(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Beverly A. Teicher, Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly ...Show all 14 authors... , Erik Harris, Anne Monks, Joel Morris; Show fewer authors
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=26428435; DOI=10.1016/j.ejca.2015.08.020
Laurens G.L. Sand, Katia Scotlandi, Dagmar Berghuis, B. Ewa Snaar-Jagalska, Piero Picci, Thomas Schmidt, Karoly Szuhai, Pancras Cornelis Wilhelmus Hogendoorn;
CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.
Eur. J. Cancer 51:2624-2633(2015)

PubMed=26979953; DOI=10.1073/pnas.1521251113; PMCID=PMC4822608
Jennifer Town, Helio Pais, Sally M. Harrison, Lucy F. Stead, Carole Bataille, Wilawan Bunjobpol, Jing Zhang, Terence Howard Rabbitts;
Exploring the surfaceome of Ewing sarcoma identifies a new and unique therapeutic target.
Proc. Natl. Acad. Sci. U.S.A. 113:3603-3608(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29464090; DOI=10.18632/oncotarget.23815; PMCID=PMC5814230
Christian Spurny, Sareetha Kailayangiri, Bianca Altvater, Silke Jamitzky, Wolfgang Hartmann, Eva Wardelmann, Andreas Ranft, Uta Dirksen, Susanne Amler, Jendrik Hardes, Maike Fluegge ...Show all 15 authors... , Jutta Meltzer, Nicole Farwick, Lea Greune, Claudia Rossig; Show fewer authors
T cell infiltration into Ewing sarcomas is associated with local expression of immune-inhibitory HLA-G.
Oncotarget 9:6536-6549(2018)

PubMed=30879952; DOI=10.1016/j.ymthe.2019.02.014; PMCID=PMC6520468
Sareetha Kailayangiri, Bianca Altvater, Stefanie Lesch, Sebastian T. Balbach, Claudia Gottlich, Johanna Kuhnemundt, Jan-Henrik Mikesch, Sonja Schelhaas, Silke Jamitzky, Jutta Meltzer, Nicole Farwick ...Show all 20 authors... , Lea Greune, Maike Fluegge, Kornelius Kerl, Holger N. Lode, Nikolai Siebert, Ingo Muller, Heike Walles, Wolfgang Hartmann, Claudia Rossig; Show fewer authors
EZH2 inhibition in Ewing sarcoma upregulates GD2 expression for targeting with gene-modified T cells.
Mol. Ther. 27:933-946(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

DOI=10.5282/edoc.27750
Martin Franz Orth;
Systematic multi-omics profiling of Ewing sarcoma cell lines.
Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany

PubMed=36476851; DOI=10.1016/j.celrep.2022.111761; PMCID=PMC10333306
Martin Franz Orth, Didier Surdez, Tobias Faehling, Anna C. Ehlers, Aruna Marchetto, Sandrine Grossetete-Lalami, Richard Volckmann, Danny A. Zwijnenburg, Julia Sophie Gerke, Sakina Zaidi, Javier Alonso ...Show all 25 authors... , Ana Sastre, Sylvain Baulande, Martin Sill, Florencia Cidre-Aranaz, Shunya Ohmura, Thomas Kirchner, Stefanie M. Hauck, Eva Reischl, Melissa Gymrek, Stefan Michael Pfister, Konstantin Strauch, Jan Koster, Olivier Delattre, Thomas Georg Philipp Grunewald; Show fewer authors
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation.
Cell Rep. 41:111761.1-111761.28(2022)

Cross-references
Cell line databases/resources cancercelllines; CVCL_7151
Cell_Model_Passport; SIDM01465
DepMap; ACH-001205
Anatomy/cell type resources BTO; BTO_0004227
CRISP screens repositories BioGRID_ORCS_Cell_line; 199
Chemistry resources ChEMBL-Cells; CHEMBL4295485
ChEMBL-Targets; CHEMBL4296499
PharmacoDB; TC32_1566_2019
PubChem_Cell_line; CVCL_7151
Encyclopedic resources Wikidata; Q54971813
Gene expression databases GEO; GSM1676322
GEO; GSM1701655
GEO; GSM1899423
GEO; GSM5359712
GEO; GSM5359713
GEO; GSM5359714
GEO; GSM5359715
GEO; GSM5359716
GEO; GSM5359717
GEO; GSM5362928
GEO; GSM5362929
GEO; GSM5362936
GEO; GSM5362937
GEO; GSM5362944
GEO; GSM5362945
GEO; GSM5364011
GEO; GSM5364012
GEO; GSM5364013
GEO; GSM5364014
GEO; GSM5364015
Polymorphism and mutation databases Cosmic; 759891
Cosmic; 1078387
Cosmic; 1084513
Cosmic; 1718432
Cosmic; 2060803
Cosmic; 2228244
Cosmic; 2294588
Progenetix; CVCL_7151
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number33