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Cellosaurus HEK293-EBNA (CVCL_6974)

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Cell line name HEK293-EBNA
Synonyms 293 c18; 293c18; HEK 293 c18; HEK-293 c18; HEK293-EBNA1; HEK-293-EBNA; HEK 293-EBNA; HEK 293 EBNA; HEK293EBNA; 293 EBNA; 293-EBNA1; 293-EBNA; 293/EBNA; 293EBNA; EBNA-293; EBNA293; 293E; HEK293E; HEK/EBNA; HEK-EBNA; HEK.EBNA; 293/EBNA-1
Accession CVCL_6974
Secondary accession CVCL_6350
Resource Identification Initiative To cite this cell line use: HEK293-EBNA (RRID:CVCL_6974)
Comments Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10852.
Characteristics: The use of recombinant protein expression vectors containing the EBV oriP in cell lines stably expressing EBV's EBNA1 protein significantly increases recombinant protein yield.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Discontinued: Thermo Fisher/Invitrogen; Catalog number R620-07.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Children:
CVCL_H365 (293 c18/DRD4)CVCL_H367 (293 c18/EP4)CVCL_A0DF (293C18P)
CVCL_E3D5 (293EBNA-B55)CVCL_D4BR (HEK293E_Clu AB1)CVCL_D4BS (HEK293E_Clu AB2)
CVCL_D4BJ (HEK293E_Clu CC1)CVCL_D4BK (HEK293E_Clu CC2)CVCL_D4BL (HEK293E_Clu CC3)
CVCL_D4BM (HEK293E_Clu CC4)CVCL_D4BN (HEK293E_Clu DD1)CVCL_E4JU (HEK293E_Clu DD1 + TL1)
CVCL_D4BP (HEK293E_Clu DD2)CVCL_D4BQ (HEK293E_Clu DD3)CVCL_D4BE (HEK293E_Clu TL1)
CVCL_D4BF (HEK293E_Clu TL2)CVCL_D4BH (HEK293E_Clu TL2+3)CVCL_D4BG (HEK293E_Clu TL3)
CVCL_D4BI (HEK293E_Clu TL4)CVCL_D4BD (HEK293E_Clu WT)CVCL_D4BT (HEK293E_Ecto short VLDLR-C-tag)
CVCL_ZJ07 (LanthaScreen Akt HEK 293E)CVCL_ZJ06 (LanthaScreen PDCD4 HEK 293E)CVCL_ZJ08 (LanthaScreen PRAS40 HEK 293E)
CVCL_W713 (VEGF-D 293 clone 11)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC=CRL-10852; CCRID=1101HUM-PUMC000152

Markers:
AmelogeninX
CSF1PO11,12
D2S133819
D3S135815,17
D5S8188,9
D7S82011,12
D8S117912,14
D13S31712 (CCRID=1101HUM-PUMC000152)
12,14 (ATCC=CRL-10852)
D16S5399
D18S5118
D19S43318
D21S1128,30.2
FGA23
Penta D9,10
Penta E7,15
TH017,9.3
TPOX11
vWA16,19

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/2/cell-lines-detail-131.html
Provider; Thermo Fisher; R620-07; https://tools.thermofisher.com/content/sfs/manuals/293ebna_man.pdf
Publications

PubMed=21356792; DOI=10.1101/pdb.prot4976
Roseanne Tom, Louis Bisson, Yves Durocher;
Culture of HEK293-EBNA1 cells for production of recombinant proteins.
CSH Protoc. 2008:Pdb.prot4976-Pdb.prot4976(2008)

DOI=10.13005/bpj/1414
Jun Yuan, Wayne Wen-Yan Xu, Snake Yu-Lin Jiang, Henry Xiao-Yu Yu, H.-Fai Poon;
The scattered twelve tribes of HEK293.
Biomed. Pharmacol. J. 11:621-623(2018)

Cross-references
Cell line collections (Providers) ATCC; CRL-10852
CLS; 300264
Cell line databases/resources CLO; CLO_0001231
CCRID; 1101HUM-PUMC000152
FCS-free; 6-2-513-1-16-12
TOKU-E; 242
TOKU-E; 3609
TOKU-E; 3672
Anatomy/cell type resources BTO; BTO_0002974
Chemistry resources ChEMBL-Cells; CHEMBL3833702
PubChem_Cell_line; CVCL_6974
Encyclopedic resources Wikidata; Q54882445
Experimental variables resources EFO; EFO_0022562
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number31