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Cellosaurus NCI-H3255 (CVCL_6831)

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Cell line name NCI-H3255
Synonyms H3255; H-3255; H3255_DA; NCIH3255
Accession CVCL_6831
Resource Identification Initiative To cite this cell line use: NCI-H3255 (RRID:CVCL_6831)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: TCGA-110-CL cell line panel.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Membrane.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Mutation; HGNC; HGNC:11998; TP53; Simple; c.560-1G>A; ClinVar=VCV000634769; Zygosity=Unspecified; Note=Splice acceptor mutation (DepMap=ACH-000109).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,03:01
HLA-BB*37:01,41:01
HLA-CC*06:02,06:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.63
Native American0
East Asian, North2.06
East Asian, South0
South Asian0
European, North59.85
European, South34.46
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_DI55 (NCI-H3255GR)
Sex of cell Female
Age at sampling 47Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2882; Cosmic-CLP=1247873; PubMed=20679594

Markers:
AmelogeninX
CSF1PO11,14
D5S81811,12
D7S82010,13
D13S31711,13
D16S5398
TH016
TPOX8,11
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=12759538; DOI=10.1159/000070299
Takashi Fujishita, Massimo Loda, Ross E. Turner, Marinshine Gentler, Tatsuhiko Kashii, Oscar S. Breathnach, Bruce E. Johnson;
Sensitivity of non-small-cell lung cancer cell lines established from patients treated with prolonged infusions of paclitaxel.
Oncology 64:399-406(2003)

PubMed=16187286; DOI=10.1002/ijc.21491
Cathie Garnis, William W. Lockwood, Emily Vucic, Ying Ge, Luc Girard, John D. Minna, Adi F. Gazdar, Stephen Lam, Calum MacAulay, Wan L. Lam;
High resolution analysis of non-small cell lung cancer cell lines by whole genome tiling path array CGH.
Int. J. Cancer 118:1556-1564(2006)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20679594; DOI=10.1093/jnci/djq279; PMCID=PMC2935474
Adi F. Gazdar, Luc Girard, William W. Lockwood, Wan L. Lam, John D. Minna;
Lung cancer cell lines as tools for biomedical discovery and research.
J. Natl. Cancer Inst. 102:1310-1321(2010)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112; PMCID=PMC3567922
Lauren Averett Byers, Jing Wang, Monique B. Nilsson, Junya Fujimoto, Pierre Saintigny, John Stuart Yordy, Uma Giri, Michael Peyton, You-Hong Fan, Li-Xia Diao, Fatemeh Masrorpour ...Show all 28 authors... , Li Shen, Wen-Bin Liu, Boris Duchemann, Praveen Tumula, Vikas Bhardwaj, James Welsh, Stephanie Weber, Bonnie S. Glisson, Neda Kalhor, Ignacio Ivan Wistuba, Luc Girard, Scott M. Lippman, Gordon B. Mills, Kevin Robert Coombes, John N. Weinstein, John D. Minna, John Victor Heymach; Show fewer authors
Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
Cancer Discov. 2:798-811(2012)

PubMed=26202522; DOI=10.1021/acs.jproteome.5b00477; PMCID=PMC4761227
Reta Birhanu Kitata, Baby Rorielyn T. Dimayacyac-Esleta, Wai-Kok Choong, Chia-Feng Tsai, Tai-Du Lin, Chih-Chiang Tsou, Shao-Hsing Weng, Yi-Ju Chen, Pan-Chyr Yang, Susan D. Arco, Alexey I. Nesvizhskii ...Show all 13 authors... , Ting-Yi Sung, Yu-Ju Chen; Show fewer authors
Mining missing membrane proteins by high-pH reverse-phase stagetip fractionation and multiple reaction monitoring mass spectrometry.
J. Proteome Res. 14:3658-3669(2015)

PubMed=26554430; DOI=10.1021/acs.analchem.5b03639
Baby Rorielyn T. Dimayacyac-Esleta, Chia-Feng Tsai, Reta Birhanu Kitata, Pei-Yi Lin, Wai-Kok Choong, Tai-Du Lin, Yi-Ting Wang, Shao-Hsing Weng, Pan-Chyr Yang, Susan D. Arco, Ting-Yi Sung, Yu-Ju Chen;
Rapid high-pH reverse phase stagetip for sensitive small-scale membrane proteomic profiling.
Anal. Chem. 87:12016-12023(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26361996; DOI=10.1016/j.jprot.2015.09.003
Kathrin Grundner-Culemann, J. Nikolaj Dybowski, Martin Klammer, Andreas Tebbe, Christoph Schaab, Henrik Daub;
Comparative proteome analysis across non-small cell lung cancer cell lines.
J. Proteomics 130:1-10(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540
Elizabeth A. McMillan, Myung-Jeom Ryu, Caroline H. Diep, Saurabh Mendiratta, Jean Rene Clemenceau, Rachel M. Vaden, Ju-Hwa Kim, Takashi Motoyaji, Kyle R. Covington, Michael Peyton, Kenneth Huffman ...Show all 40 authors... , Xiao-Feng Wu, Luc Girard, Yeojin Sung, Pei-Hsaun Chen, Prema Latha Mallipeddi, Joo Young Lee, Jordan Hanson, Sukesh Voruganti, Yunku Yu, Sunho Park, Jessica Sudderth, Christopher DeSevo, Donna M. Muzny, HarshaVardhan Doddapaneni, Adi F. Gazdar, Richard Alexander Gibbs, Tae Hyun Hwang, John Victor Heymach, Ignacio Ivan Wistuba, Kevin Robert Coombes, Noelle S. Williams, David A. Wheeler, John B. MacMillan, Ralph J. DeBerardinis, Michael G. Roth, Bruce Arthur Posner, John D. Minna, Hyun Seok Kim, Michael A. White; Show fewer authors
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2; PMCID=PMC6687785
Katharine Yu, Bin Chen, Dvir Aran, Joe Charalel, Christina Yau, Douglas M. Wolf, Laura J. van 't Veer, Atul Janardhan Butte, Ted Goldstein, Marina Sirota;
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)

PubMed=31803961; DOI=10.1002/jcb.29564; PMCID=PMC7496084
James L. Mulshine, Peter Ujhazy, Melissa Antman, Christine M. Burgess, Igor A. Kuzmin, Paul A. Bunn Jr., Bruce E. Johnson, Jack Alan Roth, Harvey I. Pass, Sheila M. Ross, Carolyn R. Aldige ...Show all 13 authors... , Ignacio Ivan Wistuba, John D. Minna; Show fewer authors
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-2882 - Discontinued
NCI-DTP; NCI-H3255
Cell line databases/resources cancercelllines; CVCL_6831
Cell_Model_Passport; SIDM00046
Cosmic-CLP; 1247873
DepMap; ACH-000109
DepMap; ACH-002137 - Discontinued
IGRhCellID; H3255_DAGEO
Anatomy/cell type resources BTO; BTO_0006539
Biological sample resources BioSample; SAMN03471808
BioSample; SAMN10988059
Chemistry resources ChEMBL-Cells; CHEMBL4295484
ChEMBL-Targets; CHEMBL4296476
GDSC; 1247873
PharmacoDB; NCIH3255_1108_2019
PubChem_Cell_line; CVCL_6831
Encyclopedic resources Wikidata; Q54908036
Experimental variables resources EFO; EFO_0003123
Gene expression databases ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM63349
GEO; GSM108847
GEO; GSM108848
GEO; GSM253411
GEO; GSM434297
GEO; GSM794371
GEO; GSM817108
GEO; GSM827356
GEO; GSM1374743
GEO; GSM1669833
Polymorphism and mutation databases Cosmic; 897734
Cosmic; 903623
Cosmic; 914952
Cosmic; 1028937
Cosmic; 1128245
Cosmic; 1146914
Cosmic; 2647633
IARC_TP53; 28350
LiGeA; CCLE_545
Progenetix; CVCL_6831
Proteomic databases PRIDE; PXD001548
PRIDE; PXD002224
PRIDE; PXD002556
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number39