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Cellosaurus NCI-H3122 (CVCL_5160)

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Cell line name NCI-H3122
Synonyms H3122; H-3122; NCIH3122
Accession CVCL_5160
Resource Identification Initiative To cite this cell line use: NCI-H3122 (RRID:CVCL_5160)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 48.5 hours (PubMed=29681454).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:427; ALK + HGNC; HGNC:1316; EML4; Name(s)=EML4-ALK; Note=E13A20 v1 fusion (PubMed=18594010; DOI=10.1101/2022.06.08.495301; DepMap=ACH-000337).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North1.36
East Asian, South0
South Asian1.48
European, North65.9
European, South31.26
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_4Y37 (NCI-H3122-CHR-A1)CVCL_AY53 (NCI-H3122-CR1)
Sex of cell Male
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): CLS=300484; Cosmic-CLP=1240190; PubMed=20679594

Markers:
AmelogeninX
CSF1PO11,12
D5S81811,12
D7S8208,12
D8S117913,15
D13S31710,12
D16S53911,12
D18S5113,16
FGA18,21
Penta D10,13
Penta E12
TH017,9.3
TPOX10
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=12759538; DOI=10.1159/000070299
Takashi Fujishita, Massimo Loda, Ross E. Turner, Marinshine Gentler, Tatsuhiko Kashii, Oscar S. Breathnach, Bruce E. Johnson;
Sensitivity of non-small-cell lung cancer cell lines established from patients treated with prolonged infusions of paclitaxel.
Oncology 64:399-406(2003)

PubMed=18594010; DOI=10.1158/1078-0432.CCR-08-0168; PMCID=PMC3025451
Jussi P. Koivunen, Craig H. Mermel, Kreshnik Zejnullahu, Carly Murphy, Eugene Lifshits, Alison J. Holmes, Hwan Geun Choi, Jhingook Kim, Derek Y. Chiang, Roman Kurt Thomas, Jinseon Lee ...Show all 21 authors... , William G. Richards, David John Sugarbaker, Christopher Thomas Ducko, Neal I. Lindeman, J. Paul Marcoux 2nd, Jeffrey Adam Engelman, Nathanael S. Gray, Charles Lee, Matthew Langer Meyerson, Pasi Antero Janne; Show fewer authors
EML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer.
Clin. Cancer Res. 14:4275-4283(2008)

PubMed=20679594; DOI=10.1093/jnci/djq279; PMCID=PMC2935474
Adi F. Gazdar, Luc Girard, William W. Lockwood, Wan L. Lam, John D. Minna;
Lung cancer cell lines as tools for biomedical discovery and research.
J. Natl. Cancer Inst. 102:1310-1321(2010)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112; PMCID=PMC3567922
Lauren Averett Byers, Jing Wang, Monique B. Nilsson, Junya Fujimoto, Pierre Saintigny, John Stuart Yordy, Uma Giri, Michael Peyton, You-Hong Fan, Li-Xia Diao, Fatemeh Masrorpour ...Show all 28 authors... , Li Shen, Wen-Bin Liu, Boris Duchemann, Praveen Tumula, Vikas Bhardwaj, James Welsh, Stephanie Weber, Bonnie S. Glisson, Neda Kalhor, Ignacio Ivan Wistuba, Luc Girard, Scott M. Lippman, Gordon B. Mills, Kevin Robert Coombes, John N. Weinstein, John D. Minna, John Victor Heymach; Show fewer authors
Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
Cancer Discov. 2:798-811(2012)

PubMed=23344087; DOI=10.1097/JTO.0b013e318283dcc0
Soyeon Kim, Tae Min Kim, Dong-Wan Kim, Heounjeong Go, Bhumsuk Keam, Se-Hoon Lee, Ja-Lok Ku, Doo Hyun Chung, Dae Seog Heo;
Heterogeneity of genetic changes associated with acquired crizotinib resistance in ALK-rearranged lung cancer.
J. Thorac. Oncol. 8:415-422(2013)

PubMed=24675041; DOI=10.1158/2159-8290.CD-13-0846; PMCID=PMC4068971
Luc Friboulet, Nan-Xin Li, Ryohei Katayama, Christian C. Lee, Justin Franklin Gainor, Adam S. Crystal, Pierre-Yves Michellys, Mark M. Awad, Noriko Yanagitani, Sungjoon Kim, AnneMarie Culazzo Pferdekamper ...Show all 24 authors... , Jie Li, Shailaja Kasibhatla, Frank Sun, Xiu-Ying Sun, Su Hua, Peter McNamara, Sidra Mahmood, Elizabeth L. Lockerman, Naoya Fujita, Makoto Nishio, Jennifer L. Harris, Alice Tsang Shaw, Jeffrey Adam Engelman; Show fewer authors
The ALK inhibitor ceritinib overcomes crizotinib resistance in non-small cell lung cancer.
Cancer Discov. 4:662-673(2014)

PubMed=26361996; DOI=10.1016/j.jprot.2015.09.003
Kathrin Grundner-Culemann, J. Nikolaj Dybowski, Martin Klammer, Andreas Tebbe, Christoph Schaab, Henrik Daub;
Comparative proteome analysis across non-small cell lung cancer cell lines.
J. Proteomics 130:1-10(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29444439; DOI=10.1016/j.celrep.2018.01.051; PMCID=PMC6343826
Tina L. Yuan, Arnaud Amzallag, Rachel Bagni, Ming Yi, Shervin Afghani, William Burgan, Nicole Fer, Leslie A. Strathern, Katie Powell, Brian Smith, Andrew Michael Waters ...Show all 30 authors... , David Alan Drubin, Ty Thomson, Rosy Liao, Patricia Greninger, Giovanna T. Stein, Ellen Murchie, Eliane Cortez, Regina K. Egan, Lauren Procter, Matthew Bess, Kwong Tai Cheng, Chih-Shia Lee, Liam Changwoo Lee, Christof Fellmann, Robert Stephens, Ji Luo, Scott William Lowe, Cyril Henri Benes, Frank McCormick; Show fewer authors
Differential effector engagement by oncogenic KRAS.
Cell Rep. 22:1889-1902(2018)

PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540
Elizabeth A. McMillan, Myung-Jeom Ryu, Caroline H. Diep, Saurabh Mendiratta, Jean Rene Clemenceau, Rachel M. Vaden, Ju-Hwa Kim, Takashi Motoyaji, Kyle R. Covington, Michael Peyton, Kenneth Huffman ...Show all 40 authors... , Xiao-Feng Wu, Luc Girard, Yeojin Sung, Pei-Hsaun Chen, Prema Latha Mallipeddi, Joo Young Lee, Jordan Hanson, Sukesh Voruganti, Yunku Yu, Sunho Park, Jessica Sudderth, Christopher DeSevo, Donna M. Muzny, HarshaVardhan Doddapaneni, Adi F. Gazdar, Richard Alexander Gibbs, Tae Hyun Hwang, John Victor Heymach, Ignacio Ivan Wistuba, Kevin Robert Coombes, Noelle S. Williams, David A. Wheeler, John B. MacMillan, Ralph J. DeBerardinis, Michael G. Roth, Bruce Arthur Posner, John D. Minna, Hyun Seok Kim, Michael A. White; Show fewer authors
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31803961; DOI=10.1002/jcb.29564; PMCID=PMC7496084
James L. Mulshine, Peter Ujhazy, Melissa Antman, Christine M. Burgess, Igor A. Kuzmin, Paul A. Bunn Jr., Bruce E. Johnson, Jack Alan Roth, Harvey I. Pass, Sheila M. Ross, Carolyn R. Aldige ...Show all 13 authors... , Ignacio Ivan Wistuba, John D. Minna; Show fewer authors
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)

DOI=10.1101/2022.06.08.495301
Marco P. Licciardello, Chi Zhang, Anh T. Le, Robert C. Doebele, Paul A. Clarke, Paul Workman;
Loss of spindly sensitizes EML4-ALK v3 lung cancer cells to HSP90 inhibitors.
bioRxiv 2022:06.08.495301-06.08.495301(2022)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) CLS; 300484
NCI-DTP; NCI-H3122
Cell line databases/resources CLO; CLO_0037033
cancercelllines; CVCL_5160
Cell_Model_Passport; SIDM00137
Cosmic-CLP; 1240190
DepMap; ACH-000337
IGRhCellID; H3122GEO
LINCS_LDP; LCL-1675
Biological sample resources BioSample; SAMN10989614
CRISP screens repositories BioGRID_ORCS_Cell_line; 962
Chemistry resources ChEMBL-Cells; CHEMBL4295436
ChEMBL-Targets; CHEMBL4296475
GDSC; 1240190
PharmacoDB; NCIH3122_1104_2019
PubChem_Cell_line; CVCL_5160
Encyclopedic resources Wikidata; Q54908028
Experimental variables resources EFO; EFO_0022675
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM171874
GEO; GSM171875
GEO; GSM253349
GEO; GSM434307
GEO; GSM794370
GEO; GSM817107
GEO; GSM1374739
GEO; GSM1374740
GEO; GSM1670238
Polymorphism and mutation databases Cosmic; 755472
Cosmic; 914951
Cosmic; 1146912
Cosmic; 2245571
Progenetix; CVCL_5160
Proteomic databases PRIDE; PXD002556
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000082
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number39