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Cellosaurus TMNK-1 (CVCL_4W79)

[Text version]
Cell line name TMNK-1
Synonyms TMNK1
Accession CVCL_4W79
Resource Identification Initiative To cite this cell line use: TMNK-1 (RRID:CVCL_4W79)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-8017.
Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-NeoR-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
Transfected with: HGNC; 11730; TERT.
Transfected with: UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Endothelial cell of hepatic sinusoid; CL=CL_1000398.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): JCRB

Markers:
AmelogeninX
CSF1PO10
D5S81811
D7S82011,13
D13S31711
D16S53910,11
TH016,7
TPOX9
vWA15,16

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Publications

PubMed=15167590; DOI=10.1097/01.TP.0000124286.82961.7E
Matsumura T., Takesue M., Westerman K.A., Okitsu T., Sakaguchi M., Fukazawa T., Totsugawa T., Noguchi H., Yamamoto S., Stolz D.B., Tanaka N., Leboulch P., Kobayashi N.
Establishment of an immortalized human-liver endothelial cell line with SV40T and hTERT.
Transplantation 77:1357-1365(2004)

Patent=WO2008066199A1
Kobayashi N., Rivas-Carrillo J.D., Tanaka N.
Method of inducing differentiation of embryonic stem cells into insulin-secreting cells, insulin-secreting cells induced by the method and use of the same.
Patent number WO2008066199A1, 05-Jun-2008

Cross-references
Cell line collections (Providers) JCRB; JCRB1564
Encyclopedic resources Wikidata; Q54972700
Entry history
Entry creation14-Dec-2015
Last entry update05-Oct-2023
Version number13