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Cellosaurus TMNK-1 (CVCL_4W79)

[Text version]
Cell line name TMNK-1
Synonyms TMNK1
Accession CVCL_4W79
Resource Identification Initiative To cite this cell line use: TMNK-1 (RRID:CVCL_4W79)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Japanese National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology; FERM BP-8017.
Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-Neo-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Endothelial cell of hepatic sinusoid; CL=CL_1000398.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Age unspecified
Category Transformed cell line
STR profile Source(s): JCRB=JCRB1564

Markers:
AmelogeninX
CSF1PO10
D5S81811
D7S82011,13
D13S31711
D16S53910,11
TH016,7
TPOX9
vWA15,16

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Publications

PubMed=15167590; DOI=10.1097/01.TP.0000124286.82961.7E
Toshihisa Matsumura, Michihiko Takesue, Karen A. Westerman, Teru Okitsu, Masakiyo Sakaguchi, Takuya Fukazawa, Toshinori Totsugawa, Hirofumi Noguchi, Shinichiro Yamamoto, Donna B. Stolz, Noriaki Tanaka ...Show all 13 authors... , Philippe Leboulch, Naoya Kobayashi; Show fewer authors
Establishment of an immortalized human-liver endothelial cell line with SV40T and hTERT.
Transplantation 77:1357-1365(2004)

Patent=WO2008066199A1
Naoya Kobayashi, Jorge David Rivas-Carrillo, Noriaki Tanaka;
Method of inducing differentiation of embryonic stem cells into insulin-secreting cells, insulin-secreting cells induced by the method and use of the same.
Patent number WO2008066199A1, 05-Jun-2008

Cross-references
Cell line collections (Providers) JCRB; JCRB1564
Encyclopedic resources Wikidata; Q54972700
Entry history
Entry creation14-Dec-2015
Last entry update14-Aug-2025
Version number18