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Cellosaurus EPC (CVCL_4361)

[Text version]
Cell line name EPC
Synonyms Epithelioma Papulosum Cyprini
Accession CVCL_4361
Resource Identification Initiative To cite this cell line use: EPC (RRID:CVCL_4361)
Comments Group: Fish cell line.
Problematic cell line: Misidentified. Originally deposited as a carp (Cyprinus carpio) cell line, is in fact a fathead minnow (Pimephales promelas) cell line (PubMed=20497291).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00302.
Doubling time: ~36 hours (ATCC=CRL-2872).
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin Pimephales promelas (Fathead minnow) (NCBI Taxonomy: 90988)
Hierarchy Children:
CVCL_E3AG (EPC-EC)CVCL_6E02 (EPCtiHSP70)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Spontaneously immortalized cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/epc-atcc-crl-2872
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; Wikipedia; -; https://de.wikipedia.org/wiki/EPC-Zellen
Publications

DOI=10.1016/S0769-2617(83)80060-4
Nikola Fijan, D. Sulimanovic, Monique Bearzotti, D. Muzinic, Lutz Oscar Zwillenberg, Stefan Chilmonczyk, Jean-Francois Vautherot, Pierre de Kinkelin;
Some properties of the Epithelioma papulosum cyprini (EPC) cell line from carp Cyprinus carpio.
Ann. Inst. Pasteur Virol. 134:207-220(1983)

DOI=10.3354/dao001209
Takahisa Kimura, Mamoru Yoshimizu, Shigeaki Gorie;
A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis.
Dis. Aquat. Organ. 1:209-217(1986)

CLPUB00103
Mamoru Yoshimizu, Masanori Kamei, S. Dirakbusarakom, Takahisa Kimura;
Fish cell lines: susceptibility to salmonid viruses.
(In book chapter) Invertebrate and fish tissue culture; Kuroda, Yukiaki & Kurstak, Edouard & Maramorosch, Karl (eds.); pp.207-217; Japan Sci. Soc.; Tokyo; Japan (1988)

DOI=10.1111/j.1365-2761.1989.tb01290.x
G. Nicolas Frerichs, Barry J. Hill, Keith Way;
Ulcerative disease rhabdovirus: cell-line susceptibility and serological comparison with other fish rhabdoviruses.
J. Fish Dis. 12:51-56(1989)

DOI=10.3147/jsfp.28.27
Roselyn D. Fernandez, Mamoru Yoshimizu, Yoshio Ezura, Takahisa Kimura;
Comparative growth response of fish cell lines in different media, temperatures, and and sodium chloride concentrations.
Fish Pathol. 28:27-34(1993)

DOI=10.3147/jsfp.30.23
Myung-Joo Oh, Mamoru Yoshimizu, Takahisa Kimura, Yoshio Ezura;
A new virus isolated from salmonid fish.
Fish Pathol. 30:23-32(1995)

PubMed=11673072; DOI=10.1016/S1383-5718(01)00268-6
Ulrike Kammann, Markus Bunke, Hans Steinhart, Norbert Theobald;
A permanent fish cell line (EPC) for genotoxicity testing of marine sediments with the comet assay.
Mutat. Res. 498:67-77(2001)

DOI=10.1016/S1873-0140(05)80005-0
Niels C. Bols, Vivian R. Dayeh, Lucy E.J. Lee, Kristin Schirmer;
Use of fish cell lines in the toxicology and ecotoxicology of fish Piscine cell lines in environmental toxicology.
(In book chapter) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen, Thomas P. & Moon, Thomas W. (eds.); pp.43-84; Elsevier; Amsterdam; Netherlands (2005)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20497291; DOI=10.1111/j.1365-2761.2010.01165.x
James R. Winton, William N. Batts, Pierre de Kinkelin, Monique LeBerre, Michel Bremont, Nikola Fijan;
Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells.
J. Fish Dis. 33:701-704(2010)

PubMed=33053677; DOI=10.3390/pathogens9100833; PMCID=PMC7601784
Phuc H. Pham, Ehab Misk, Fotini Papazotos, Ginny Jones, Mark P. Polinski, Elena Contador, Spencer Russell, Kyle A. Garver, John S. Lumsden, Niels C. Bols;
Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1) amplification: identification of the non-supportive PRV-1 invitrome.
Pathogens 9:833.1-833.25(2020)

PubMed=34382581; DOI=10.3354/dao03615
Takafumi Ito, Takashi Kamaishi;
Japanese amberjack Seriola quinqueradiata and red sea bream Pagrus major susceptibility to infectious hematopoietic necrosis virus (IHNV) isolate.
Dis. Aquat. Organ. 146:1-8(2021)

Cross-references
Cell line collections (Providers) ATCC; CRL-2872
CCLV; CCLV-RIE 0173
CCTCC; GDC0174
CCTCC; GDC0182
ECACC; 93120820
IZSLER; BS CL 41
Cell line databases/resources CLO; CLO_0002940
CLDB; cl1167
CLDB; cl1168
Biological sample resources BioSample; SAMN03152008
Encyclopedic resources Wikidata; Q26882920
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number27