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Cellosaurus NCI-H125 (CVCL_3968)

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Cell line name NCI-H125
Synonyms H125; H-125; NCI-Hut 125; NCI-HUT-125; NCI-HUT 125; HUT-125; HUT 125; HUT125
Accession CVCL_3968
Resource Identification Initiative To cite this cell line use: NCI-H125 (RRID:CVCL_3968)
Comments Part of: MD Anderson Cell Lines Project.
Population: African American.
Doubling time: 39 hours (Note=In RPMI 1640 + 10% FBS), 39 hours (Note=In ACL-3), 40 hours (Note=In ACL-3 + BSA) (PubMed=3940644).
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Hypodermis; UBERON=UBERON_0002072.
Sequence variations
Disease Lung adenosquamous carcinoma (NCIt: C9133)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A570 (NCI-H513)
Sex of cell Male
Age at sampling 61Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-5801; CCRID=1102HUM-NIFDC00022; PubMed=20679594

Markers:
AmelogeninX (ATCC=CRL-5801; CCRID=1102HUM-NIFDC00022)
X,Y (PubMed=20679594)
CSF1PO7
D3S135816,17
D5S81810
D7S82010
D8S117915
D13S31711
D16S5395,12
D18S5120
D21S1129,32.2
FGA22,24
Penta D11,12
Penta E7
TH017
TPOX8,9
vWA17

Run an STR similarity search on this cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=6270685; DOI=10.1073/pnas.78.7.4591; PMCID=PMC319839
Frank Cuttitta, Steven T. Rosen, Adi F. Gazdar, John D. Minna;
Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.
Proc. Natl. Acad. Sci. U.S.A. 78:4591-4595(1981)

PubMed=6272398; DOI=10.1126/science.6272398
Terry W. Moody, Candace Beebe Pert, Adi F. Gazdar, Desmond Niall Carney, John D. Minna;
High levels of intracellular bombesin characterize human small-cell lung carcinoma.
Science 214:1246-1248(1981)

PubMed=6148444; DOI=10.1093/jnci/73.4.801
George Morstyn, Angelo Russo, Desmond Niall Carney, Essam Karawya, Samuel H. Wilson Jr., James B. Mitchell;
Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.
J. Natl. Cancer Inst. 73:801-807(1984)

PubMed=6713399
Steven T. Rosen, James L. Mulshine, Frank Cuttitta, Joseph Fedorko, Desmond Niall Carney, Adi F. Gazdar, John D. Minna;
Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.
Cancer Res. 44:2052-2061(1984)

PubMed=3940644
Martin Brower, Desmond Niall Carney, Herbert Kazuto Oie, Adi F. Gazdar, John D. Minna;
Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.
Cancer Res. 46:798-806(1986)

PubMed=2473086; DOI=10.1242/jcs.91.1.91
Jos L.V. Broers, Mirjam Klein Rot, Tineke Oostendorp, Gerold Bepler, Lou F.M.H. de Leij, Desmond Niall Carney, Gijsbert Peter Vooijs, Frans C.S. Ramaekers;
Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines.
J. Cell Sci. 91:91-108(1988)

PubMed=3335022
Michael C. Alley, Dominic A. Scudiero, Anne Monks, Miriam L. Hursey, Maciej J. Czerwinski, Donald L. Fine, Betty Jane Abbott, Joseph G. Mayo, Robert H. Shoemaker, Michael R. Boyd;
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=2388294; DOI=10.1093/jnci/82.17.1420
Theodore L. McLemore, Charles L. Litterst, Bruno P. Coudert, Mark C. Liu, Walter C. Hubbard, Steven Adelberg, Maciej J. Czerwinski, Noreen A. McMahon, Joseph C. Eggleston, Michael R. Boyd;
Metabolic activation of 4-ipomeanol in human lung, primary pulmonary carcinomas, and established human pulmonary carcinoma cell lines.
J. Natl. Cancer Inst. 82:1420-1426(1990)

PubMed=1845952; DOI=10.1002/1097-0142(19910201)67:3<619::AID-CNCR2820670317>3.0.CO;2-Y
Jos L.V. Broers, Eddy P. Mijnheere, Mirjam Klein Rot, Gert Schaart, Alfred Sijlmans, Otto C. Boerman, Frans C.S. Ramaekers;
Novel antigens characteristic of neuroendocrine malignancies.
Cancer 67:619-633(1991)

PubMed=1311061
Tetsuya Mitsudomi, Seth M. Steinberg, Marion M. Nau, David P. Carbone, Domenico D'Amico, Sara M. Bodner, Herbert Kazuto Oie, Ritva Ilona Linnoila, James L. Mulshine, John D. Minna, Adi F. Gazdar;
p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features.
Oncogene 7:171-180(1992)

PubMed=1320893; DOI=10.1159/000163712
Marken A.E. Verbeeck, Jack P.M. Elands, Lou F.M.H. de Leij, Charles H.C.M. Buys, Desmond Niall Carney, Gerold Bepler, Anton J.M. Roebroek, Wim J.M. van de Ven, J. Peter H. Burbach;
Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines.
Pathobiology 60:136-142(1992)

PubMed=8806092; DOI=10.1002/jcb.240630505
Ruby M. Phelps, Bruce E. Johnson, Daniel C. Ihde, Adi F. Gazdar, David P. Carbone, Patrick R. McClintock, Ritva Ilona Linnoila, Mary J. Matthews, Paul A. Bunn Jr., Desmond Niall Carney, John D. Minna, James L. Mulshine;
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. Suppl. 24:32-91(1996)

PubMed=9649128; DOI=10.1038/bjc.1998.361; PMCID=PMC2150402
Seung Yi, Jing-Ru Chen, Jean Viallet, Ralph H. Schwall, T. Nakamura, Ming-Sound Tsao;
Paracrine effects of hepatocyte growth factor/scatter factor on non-small-cell lung carcinoma cell lines.
Br. J. Cancer 77:2162-2170(1998)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=11369051; DOI=10.1016/S0165-4608(00)00363-0
Catherine Luk, Ming-Sound Tsao, Jane Bayani, Frances Alice Shepherd, Jeremy Andrew Squire;
Molecular cytogenetic analysis of non-small cell lung carcinoma by spectral karyotyping and comparative genomic hybridization.
Cancer Genet. Cytogenet. 125:87-99(2001)

PubMed=12820372
Michael Bergqvist, Daniel Brattstrom, Joachim Gullbo, Patrik Hesselius, Ola Brodin, Gunnar Wagenius;
p53 status and its in vitro relationship to radiosensitivity and chemosensitivity in lung cancer.
Anticancer Res. 23:1207-1212(2003)

PubMed=20679594; DOI=10.1093/jnci/djq279; PMCID=PMC2935474
Adi F. Gazdar, Luc Girard, William W. Lockwood, Wan L. Lam, John D. Minna;
Lung cancer cell lines as tools for biomedical discovery and research.
J. Natl. Cancer Inst. 102:1310-1321(2010)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540
Elizabeth A. McMillan, Myung-Jeom Ryu, Caroline H. Diep, Saurabh Mendiratta, Jean Rene Clemenceau, Rachel M. Vaden, Ju-Hwa Kim, Takashi Motoyaji, Kyle R. Covington, Michael Peyton, Kenneth Huffman ...Show all 40 authors... , Xiao-Feng Wu, Luc Girard, Yeojin Sung, Pei-Hsaun Chen, Prema Latha Mallipeddi, Joo Young Lee, Jordan Hanson, Sukesh Voruganti, Yunku Yu, Sunho Park, Jessica Sudderth, Christopher DeSevo, Donna M. Muzny, HarshaVardhan Doddapaneni, Adi F. Gazdar, Richard Alexander Gibbs, Tae Hyun Hwang, John Victor Heymach, Ignacio Ivan Wistuba, Kevin Robert Coombes, Noelle S. Williams, David A. Wheeler, John B. MacMillan, Ralph J. DeBerardinis, Michael G. Roth, Bruce Arthur Posner, John D. Minna, Hyun Seok Kim, Michael A. White; Show fewer authors
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)

PubMed=31803961; DOI=10.1002/jcb.29564; PMCID=PMC7496084
James L. Mulshine, Peter Ujhazy, Melissa Antman, Christine M. Burgess, Igor A. Kuzmin, Paul A. Bunn Jr., Bruce E. Johnson, Jack Alan Roth, Harvey I. Pass, Sheila M. Ross, Carolyn R. Aldige ...Show all 13 authors... , Ignacio Ivan Wistuba, John D. Minna; Show fewer authors
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)

Cross-references
Cell line collections (Providers) ATCC; CRL-5801 - Discontinued
KCLB; 90125 - Discontinued
Cell line databases/resources cancercelllines; CVCL_3968
CCRID; 1102HUM-NIFDC00022
Cell_Model_Passport; SIDM00106
Anatomy/cell type resources BTO; BTO_0003010
Biological sample resources BioSample; SAMN03471737
Chemistry resources ChEMBL-Cells; CHEMBL3307888
ChEMBL-Targets; CHEMBL614159
PubChem_Cell_line; CVCL_3968
Encyclopedic resources Wikidata; Q54907780
Experimental variables resources EFO; EFO_0003116
Gene expression databases GEO; GSM784259
GEO; GSM794271
GEO; GSM817067
Polymorphism and mutation databases Cosmic; 844593
Cosmic; 877401
Cosmic; 931373
Cosmic; 1028943
Cosmic; 1032431
Cosmic; 1152496
Cosmic; 2560225
IARC_TP53; 486
Progenetix; CVCL_3968
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number39