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Cellosaurus RKO-AS45-1 (CVCL_3786)

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Cell line name RKO-AS45-1
Synonyms RKO-AS-45-1; RKOAS451; RKO_AS45
Accession CVCL_3786
Resource Identification Initiative To cite this cell line use: RKO-AS45-1 (RRID:CVCL_3786)
Comments Characteristics: Expresses a high level of GADD45A in an antisense orientation. Blocks de novo synthesis of GADD45A in response to DNA damage (PubMed=7973727).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:4095; GADD45A (Note=In antisense orientation).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Omics: Transcriptomics; Microarray.
Misspelling: RKO-AS4; Cosmic=1187316.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0504 (RKO)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2579

Markers:
AmelogeninX
CSF1PO8,11
D5S81811,13
D7S8208,10
D13S3178,11
D16S53912.1,13
TH016,10
TPOX10,11
vWA16,24

Run an STR similarity search on this cell line
Publications

PubMed=7973727; DOI=10.1126/science.7973727
Martin L. Smith, I-Tsuen Chen, Qi-Min Zhan, Insoo Bae, Chaw-Yuan Chen, Tona Morgan Gilmer, Michael Barry Kastan, Patrick M. O'Connor, Albert J. Fornace Jr.;
Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.
Science 266:1376-1380(1994)

PubMed=8631022
Insoo Bae, Martin L. Smith, M. Saeed Sheikh, Qi-Min Zhan, Dominic A. Scudiero, Stephen H. Friend, Patrick M. O'Connor, Albert J. Fornace Jr.;
An abnormality in the p53 pathway following gamma-irradiation in many wild-type p53 human melanoma lines.
Cancer Res. 56:840-847(1996)

Cross-references
Cell line collections (Providers) ATCC; CRL-2579
CLS; 305146
Cell line databases/resources CLO; CLO_0008826
cancercelllines; CVCL_3786
CCRID; 3101HUMTCHu117
Anatomy/cell type resources BTO; BTO_0001892
Biological sample resources BioSample; SAMN03471685
Encyclopedic resources Wikidata; Q54950557
Gene expression databases GEO; GSM206539
GEO; GSM274767
GEO; GSM274768
Polymorphism and mutation databases Cosmic; 1187316
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number30