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Cellosaurus NL20 (CVCL_3756)

[Text version]
Cell line name NL20
Synonyms NL-20
Accession CVCL_3756
Resource Identification Initiative To cite this cell line use: NL20 (RRID:CVCL_3756)
Comments Population: Caucasian.
Doubling time: ~48 hours (PubMed=1522038); ~24 hours (PBCF).
Karyotypic information: 43,X,-X,-5,-7,-15,del(4)(p14),del(6)(q21-q25),i(14q),18p+,21p+,+i(5q),+der(15)t(7p;15q) (PubMed=1522038).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C5B5 (NL20T)
Originate from same individual CVCL_C5AZ ! NL20SV
Sex of cell Female
Age at sampling 20Y
Category Transformed cell line
STR profile Source(s): ATCC=CRL-2503

Markers:
AmelogeninX
CSF1PO11
D2S133821,24
D3S135814,17
D5S81812
D7S82012
D8S117912,13
D13S31711,12
D16S53912
D18S5114
D19S43313,15.2
D21S1129,31.2
FGA22
Penta D9,11
Penta E6,16
TH016,7
TPOX9,11
vWA14,17

Run an STR similarity search on this cell line
Web pages Info; Synapse; PS-ON; https://www.synapse.org/#!Synapse:syn31544547
Publications

PubMed=1522038; DOI=10.1007/BF02634125
Joan H. Schiller, Gerard N. Bittner, Terry DeWayne Oberley, Ching-Hai Kao, Chuck Harris, Lorraine Faxon Meisner;
Establishment and characterization of a SV40 T-antigen immortalized human bronchial epithelial cell line.
In Vitro Cell. Dev. Biol. Anim. 28:461-464(1992)

PubMed=21566947; DOI=10.3892/ijo.4.2.461
Joan H. Schiller, Linda Sabatini, Gerard N. Bittner, Charles L. Pinkerman, Jane Mayotte, Mark L. Levitt, Lorraine Faxon Meisner;
Phenotypic, molecular and genetic-characterization of transformed human bronchial epithelial-cell strains.
Int. J. Oncol. 4:461-470(1994)

PubMed=9216860; DOI=10.1159/000227713
Jay L. Wittenkeller, Barry Storer, Gerard N. Bittner, Joan H. Schiller;
Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative.
Oncology 54:335-341(1997)

Cross-references
Cell line collections (Providers) ATCC; CRL-2503
BCRC; 60361
Cell line databases/resources CLO; CLO_0008179
Biological sample resources BioSample; SAMN03471599
BioSample; SAMN05292446
Encyclopedic resources Wikidata; Q54930686
Gene expression databases GEO; GSM185872
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number16