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Cellosaurus ASH-3 (CVCL_2856)

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Cell line name ASH-3
Synonyms ASH3
Accession CVCL_2856
Resource Identification Initiative To cite this cell line use: ASH-3 (RRID:CVCL_2856)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: 84 hours (PubMed=11686581); 43-47 hours (Note=Lot 12072015) (JCRB=JCRB1073).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Thyroid gland; UBERON=UBERON_0002046.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Unspecified (PubMed=23833040).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Arg (c.181A>G); Zygosity=Heterozygous (PubMed=30737244; Cosmic-CLP=1290722; DepMap=ACH-001443).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Phe241fs (c.724dupG); Zygosity=Homozygous (PubMed=30737244; Cosmic-CLP=1290722; DepMap=ACH-001443).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Heterozygous; Note=In promoter (PubMed=23833040; PubMed=30737244).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Arg (c.536A>G); ClinVar=VCV000376606; Zygosity=Homozygous (PubMed=30737244; Cosmic-CLP=1290722; DepMap=ACH-001443).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.08
East Asian, North79.16
East Asian, South20.41
South Asian0.34
European, North0
European, South0
Disease Thyroid gland anaplastic carcinoma (NCIt: C3878)
Anaplastic thyroid carcinoma (ORDO: Orphanet_142)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 68Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=1290722; DepMap=ACH-001443; JCRB=JCRB1073; PubMed=25877200; PubMed=30737244

Markers:
AmelogeninX
CSF1PO10,14
D2S133819,23
D3S135815
D5S81811,13
D7S82011
D8S117916
D13S31712
D16S5399
D18S5113,17
D19S43313,15.2
D21S1128
FGA20,22
Penta D10,11
Penta E5
TH019
TPOX8,11
vWA16,17

Run an STR similarity search on this cell line
Publications

PubMed=11686581; DOI=10.1016/S0753-3322(01)00087-7
Morimasa Sekiguchi, Yasuko Shiroko, Tsutomu Arai, Tohru Kishino, Isamu Sugawara, Takashi Kusakabe, Tomoyuki Suzuki, Tomoyuki Yamashita, Takao Obara, Kunihiko Ito, Kenichiro Hasumi;
Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines.
Biomed. Pharmacother. 55:466-474(2001)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=23833040; DOI=10.1210/jc.2013-2383; PMCID=PMC3763971
Inigo Landa, Ian Ganly, Timothy A. Chan, Norisato Mitsutake, Michiko Matsuse, Tihana Ibrahimpasic, Ronald A. Ghossein, James Alexander Fagin;
Frequent somatic TERT promoter mutations in thyroid cancer: higher prevalence in advanced forms of the disease.
J. Clin. Endocrinol. Metab. 98:E1562-E1566(2013)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30737244; DOI=10.1158/1078-0432.CCR-18-2953; PMCID=PMC6522280
Inigo Landa, Nikita Pozdeyev, Christopher T. Korch, Laura A. Marlow, Robert Christian Smallridge, John Alton Copland 3rd, Ying C. Henderson, Stephen Y. Lai, Gary L. Clayman, Naoyoshi Onoda, Aik-Choon Tan ...Show all 16 authors... , Maria E.R. Garcia-Rendueles, Jeffrey A. Knauf, Bryan R. Haugen, James Alexander Fagin, Rebecca E. Schweppe; Show fewer authors
Comprehensive genetic characterization of human thyroid cancer cell lines: a validated panel for preclinical studies.
Clin. Cancer Res. 25:3141-3151(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB1073
JCRB; NIHS0269 - Discontinued
Cell line databases/resources CLO; CLO_0037151
cancercelllines; CVCL_2856
Cell_Model_Passport; SIDM00491
Cosmic-CLP; 1290722
DepMap; ACH-001443
LINCS_LDP; LCL-1682
Biological sample resources BioSample; SAMN03470933
Chemistry resources GDSC; 1290722
PharmacoDB; ASH3_65_2019
Encyclopedic resources Wikidata; Q54750715
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM827189
GEO; GSM1669602
Polymorphism and mutation databases Cosmic; 2054110
Progenetix; CVCL_2856
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number35