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Cellosaurus MT-2 (CVCL_2631)

[Text version]
Cell line name MT-2
Synonyms MT2; MT-2J; MT-2Org
Accession CVCL_2631
Resource Identification Initiative To cite this cell line use: MT-2 (RRID:CVCL_2631)
Comments Population: Japanese.
Characteristics: Was established by co-cultivation of cells from a 45 year old female T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542).
Virology: HTLV-1 producing cell line. Contains 11 integrated HTLV-1 proviruses: one complete proviral genome, six copies of a provirus with an internal deletion of 5.3 kb, one copy of a provirus with an internal deletion of 3.9 kb, one copy of a provirus with a 6.2 kB deletion from 5'LTR to position 6257 and two copies of a provirus composed of LTR only.
Virology: Susceptible to infection by hepatitis C virus (HCV) (PubMed=7832798).
Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Cell type: Regulatory T-cell; CL=CL_0000815.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_0D79 (CB58/MT2)CVCL_0D80 (CB59/MT2)CVCL_C0YR (MT-2CB1)
CVCL_C0YS (MT-2CR1)
Sex of cell Male
Age at sampling <1D
Category Transformed cell line
STR profile Source(s): JCRB=JCRB1210; PubMed=28357668

Markers:
AmelogeninX,Y
CSF1PO10,11
D3S135816,17
D5S81813
D7S8208,13
D8S117911,14
D13S3179,13
D16S5399,11
D18S5112,14
D21S1129,30
FGA23,24
Penta D10,11
Penta E15,16
TH016,10
TPOX10,11
vWA16

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-576.html
Publications

PubMed=6275274; DOI=10.1038/294770a0
Isao Miyoshi, Ichiro Kubonishi, Shizuo Yoshimoto, Tadaatsu Akagi, Yuji Ohtsuki, Yukimasa Shiraishi, Kinya Nagata, Yorio Hinuma;
Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells.
Nature 294:770-771(1981)

PubMed=6281119
Isao Miyoshi, Ichiro Kubonishi, Shizuo Yoshimoto, Yukimasa Shiraishi;
A T-cell line derived from normal human cord leukocytes by co-culturing with human leukemic T-cells.
Gann 72:978-981(1981)

CLPUB00542
Isao Miyoshi, Hirokuni Taguchi, Ichiro Kubonishi, Shizuo Yoshimoto, Yuji Ohtsuki, Yukimasa Shiraishi, Tadaatsu Akagi;
Type C virus-producing cell lines derived from adult T cell leukemia.
Gann Monogr. Cancer Res. 28:219-228(1982)

PubMed=6198871; DOI=10.18926/AMO/32400
Takuzo Oda, Sekiko Watanabe, Takashi Nakamura;
Type C virus particles produced in human T-cell lines derived from acute lymphoblastic leukemia and a leukemic T-lymphoid malignancy.
Acta Med. Okayama 37:529-533(1983)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 Carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3001699; DOI=10.1073/pnas.82.24.8359; PMCID=PMC390915
Tetsuyuki Kiyokawa, Motoharu Seiki, Shintaro Iwashita, Kenichi Imagawa, Fumio Shimizu, Mitsuaki Yoshida;
p27x-III and p21x-III, proteins encoded by the pX sequence of human T-cell leukemia virus type I.
Proc. Natl. Acad. Sci. U.S.A. 82:8359-8363(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=3003193; DOI=10.4049/jimmunol.136.4.1322
John C. Lee, Linda Rebar, Peter Young, Francis W. Ruscetti, Nabil Hanna, George Poste;
Identification and characterization of a human T cell line-derived lymphokine with MAF-like activity distinct from interferon-gamma.
J. Immunol. 136:1322-1328(1986)

PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t
Satoshi Orita, Akihiko Saiga, Shin Takagi, Tatsuro Tanaka, Koichi Okumura, Yuko Aono, Yorio Hinuma, Hisanaga Igarashi;
A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein.
FEBS Lett. 295:127-134(1991)

PubMed=7662981; DOI=10.1182/blood.V86.6.2350.bloodjournal8662350
Kazunori Imada, Akifumi Takaori-Kondo, Tsuyoshi Akagi, Kunitada Shimotohno, Kazuo Sugamura, Toshio Hattori, Hirohiko Yamabe, Minoru Okuma, Takashi Uchiyama;
Tumorigenicity of human T-cell leukemia virus type I-infected cell lines in severe combined immunodeficient mice and characterization of the cells proliferating in vivo.
Blood 86:2350-2357(1995)

PubMed=7832798; DOI=10.1006/bbrc.1995.1123
Nobuyuki Kato, Takahide Nakazawa, Tetsuya Mizutani, Kunitada Shimotohno;
Susceptibility of human T-lymphotropic virus type I infected cell line MT-2 to hepatitis C virus infection.
Biochem. Biophys. Res. Commun. 206:863-869(1995)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x; PMCID=PMC5920998
Nobuhiro Kawasaki, Yoshinobu Matsuo, Tadashi Yoshino, Hiroyuki Yanai, Takashi Oka, Norihiro Teramoto, Cao Liu, Eisaku Kondo, Jun Minowada, Tadaatsu Akagi;
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588
Noriko Hosoya, Akira Hangaishi, Seishi Ogawa, Kiyoshi Miyagawa, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai;
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=12920029; DOI=10.1182/blood-2003-04-1043
Hye-Kyung Chung, Howard A. Young, Peter Kin Cho Goon, Gisela Heidecker, Gerald Lee Princler, Osamu Shimozato, Graham P. Taylor, Charles R.M. Bangham, David Daniel Derse;
Activation of interleukin-13 expression in T cells from HTLV-1-infected individuals and in chronically infected cell lines.
Blood 102:4130-4136(2003)

PubMed=15638862; DOI=10.1111/j.1365-2141.2004.05289.x
Hiroo Hasegawa, Yasuaki Yamada, Hitomi Harasawa, Tomohiro Tsuji, Ken Murata, Kazuyuki Sugahara, Kazuto Tsuruda, Shuichi Ikeda, Yoshitaka Imaizumi, Masao Tomonaga, Masoto Masuda ...Show all 13 authors... , Nobuyuki Takasu, Shimeru Kamihira; Show fewer authors
Sensitivity of adult T-cell leukaemia lymphoma cells to tumour necrosis factor-related apoptosis-inducing ligand.
Br. J. Haematol. 128:253-265(2005)

PubMed=16850164; DOI=10.1007/s10495-006-9235-4
Yoshie Miura, Yasumitsu Nishimura, Hironobu Katsuyama, Megumi Maeda, Hiroaki Hayashi, Mao-Long Dong, Fuminori Hyodoh, Masafumi Tomita, Yoshinobu Matsuo, Ayuko Uesaka, Kozo Kuribayashi ...Show all 14 authors... , Takashi Nakano, Takumi Kishimoto, Takemi Otsuki; Show fewer authors
Involvement of IL-10 and Bcl-2 in resistance against an asbestos-induced apoptosis of T cells.
Apoptosis 11:1825-1835(2006)

PubMed=19220422; DOI=10.1111/j.1600-0609.2009.01211.x
Shimeru Kamihira, Chiharu Terada, Daisuke Sasaki, Katsunori Yanagihara, Kunihiro Tsukasaki, Hiroo Hasegawa, Yasuaki Yamada;
Aberrant p53 protein expression and function in a panel of hematopoietic cell lines with different p53 mutations.
Eur. J. Haematol. 82:301-307(2009)

PubMed=22608188; DOI=10.1016/j.scitotenv.2012.04.043
Megumi Maeda, Shoko Yamamoto, Ying Chen, Naoko Kumagai-Takei, Hiroaki Hayashi, Hidenori Matsuzaki, Suni Lee, Tamayo Hatayama, Naomi Miyahara, Minako Katoh, Juni-ichi Hiratsuka ...Show all 13 authors... , Yasumitsu Nishimura, Takemi Otsuki; Show fewer authors
Resistance to asbestos-induced apoptosis with continuous exposure to crocidolite on a human T cell.
Sci. Total Environ. 429:174-182(2012)

PubMed=25544500; DOI=10.1038/cmi.2014.123; PMCID=PMC4711675
Ryoko Hamano, Xue-Qiang Wu, Yi-Tao Wang, Joost J. Oppenheim, Xin Chen;
Characterization of MT-2 cells as a human regulatory T cell-like cell line.
Cell. Mol. Immunol. 12:780-782(2015)

PubMed=26940204; DOI=10.1007/s13577-016-0136-8
Yuuki Hashikura, Kazumi Umeki, Kunihiko Umekita, Hajime Nomura, Ikuo Yamamoto, Hiroo Hasegawa, Katsunori Yanagihara, Akihiko Okayama;
The diversity of the structure and genomic integration sites of HTLV-1 provirus in MT-2 cell lines.
Hum. Cell 29:122-129(2016)

PubMed=28357668; DOI=10.1007/978-1-4939-6872-5_11
Vittoria Raimondi, Sonia Anna Minuzzo, Vincenzo Ciminale, Donna M. D'Agostino;
STR profiling of HTLV-1-infected cell lines.
Methods Mol. Biol. 1582:143-154(2017)

PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783
Setsuko Shioda, Fumio Kasai, Ken Watanabe, Kohei Kawakami, Azusa Ohtani, Masashi Iemura, Midori Ozawa, Akemi Arakawa, Noriko Hirayama, Eiko Kawaguchi, Tomoko Tano ...Show all 15 authors... , Sayaka Miyata, Motonobu Satoh, Norio Shimizu, Arihiro Kohara; Show fewer authors
Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.
R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=32187573; DOI=10.1016/j.envint.2020.105654
Shoko Yamamoto, Suni Lee, Hidenori Matsuzaki, Naoko Kumagai-Takei, Kei Yoshitome, Nagisa Sada, Yurika Shimizu, Tatsuo Ito, Yasumitsu Nishimura, Takemi Otsuki;
Enhanced expression of nicotinamide nucleotide transhydrogenase (NNT) and its role in a human T cell line continuously exposed to asbestos.
Environ. Int. 138:105654.1-105654.13(2020)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-237
ECACC; 08081401
ECACC; 93121518 - Discontinued
JCRB; JCRB1210
JCRB; NIHS0527 - Discontinued
Cell line databases/resources CLO; CLO_0007888
CLDB; cl3596
CLDB; cl3597
Anatomy/cell type resources BTO; BTO_0005492
Biological sample resources BioSample; SAMN03472437
Chemistry resources ChEMBL-Cells; CHEMBL3307558
ChEMBL-Targets; CHEMBL614184
PubChem_Cell_line; CVCL_2631
Encyclopedic resources Wikidata; Q54906935
Experimental variables resources EFO; EFO_0022393
Polymorphism and mutation databases Cosmic; 919158
IARC_TP53; 27003
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number35