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Cellosaurus HSG (CVCL_2517)

[Text version]
Cell line name HSG
Synonyms Human Salivary Gland
Accession CVCL_2517
Resource Identification Initiative To cite this cell line use: HSG (RRID:CVCL_2517)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=20143388; PubMed=29923277; CelloPub=CLPUB00653). Originally thought to originate from a 54 year old male irradiated submandibular salivary gland.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00313.
Population: African American.
Doubling time: 29 hours (DOI=10.2330/joralbiosci1965.34.164).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Proteomics; 2DE-MS.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_A047 (HSG-AZA1)CVCL_A048 (HSG-AZA3)CVCL_WL39 (HSG-S6)
CVCL_WL38 (HSG-S8)CVCL_2951 (HSGc-C5)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): CelloPub=CLPUB00653; JCRB=CHB0004; PubMed=29923277

Markers:
AmelogeninX
CSF1PO9
D3S135815,18
D5S81810,11 (CelloPub=CLPUB00653; PubMed=29923277)
11 (JCRB=CHB0004)
D7S8208,12
D8S117912,13
D13S31712,13.3
D16S5399,10
D18S5116
D21S1127,28
FGA21
Penta D8,15
Penta E7,17
TH017
TPOX8,12
vWA16,18,19

Run an STR similarity search on this cell line
Web pages Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=HSG
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=7248901; DOI=10.1002/1097-0142(19810801)48:3<745::AID-CNCR2820480314>3.0.CO;2-7
Kanemitsu Shirasuna, Mitsunobu Sato, Tadashi Miyazaki;
A neoplastic epithelial duct cell line established from an irradiated human salivary gland.
Cancer 48:745-752(1981)

PubMed=2440567
Mitsunobu Sato, Masayuki Azuma, Yoshio Hayashi, Hideo Yoshida, Tetsuo Yanagawa, Yoshiaki Yura;
5-azacytidine induction of stable myoepithelial and acinar cells from a human salivary intercalated duct cell clone.
Cancer Res. 47:4453-4459(1987)

DOI=10.2330/joralbiosci1965.34.164
Setsuko Hatakeyama, Atsumi Suzuki;
Isolation of salivary adenocarcinoma cell's subclone with in vivo activity of bone and cartilage fomation in serum-free culture.
Jpn. J. Oral Biol. 34:164-168(1992)

PubMed=9183635
Minoru Ota, Seiko Kyakumoto, Nobuko Sato;
Proliferation and differentiation of human salivary gland adenocarcinoma cell line HSG.
Hum. Cell 9:79-88(1996)

DOI=10.1007/0-306-46872-7_9
Mitsunobu Sato;
Salivary gland tumors.
(In book chapter) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.161-178; Kluwer Academic Publishers; New York; USA (1999)

PubMed=16391823; DOI=10.3892/ijmm.17.2.253
Atsushi Kasamatsu, Katsuhiro Uzawa, Dai Nakashima, Yukinao Kouzu, Yosuke Endo, Hirofumi Koike, Hidetaka Yokoe, Koji Harada, Mitsunobu Sato, Hideki Tanzawa;
A proteomics approach to characterizing human submandibular gland cell lines by fluorescent two-dimensional differential in-gel electrophoresis.
Int. J. Mol. Med. 17:253-260(2006)

PubMed=17390010; DOI=10.3892/ijo.30.5.1089
Hitoshi Kawamata, Fumie Omotehara, Koh-ichi Nakashiro, Daisuke Uchida, Yasuhiro Shinagawa, Masatsugu Tachibana, Yutaka Imai, Takahiro Fujimori;
Oncogenic mutation of the p53 gene derived from head and neck cancer prevents cells from undergoing apoptosis after DNA damage.
Int. J. Oncol. 30:1089-1097(2007)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

CLPUB00653
Li-Chieh Lin;
A new tool in human salivary gland research: the SMG-hu-1 cell line.
Thesis MDSc (2017); McGill University Montreal; Montreal; Canada

PubMed=29923277; DOI=10.1111/odi.12920
Li-Chieh Lin, Osama Elkashty, Murali Ramamoorthi, Nathalie Trinh, You-Nan Liu, Gulshan Sunavala-Dossabhoy, Thomas John F. Pranzatelli, Drew G. Michael, Clara Chivasso, Jason Perret, John A. Chiorini ...Show all 13 authors... , Christine Delporte, Simon D. Tran; Show fewer authors
Cross-contamination of the human salivary gland HSG cell line with HeLa cells: a STR analysis study.
Oral Dis. 24:1477-1483(2018)

Cross-references
Cell line collections (Providers) ECACC; 95031024
JCRB; CHB0004 - Discontinued
Cell line databases/resources CLO; CLO_0004263
Biological sample resources BioSample; SAMN03151712
BioSample; SAMN03471295
Encyclopedic resources Wikidata; Q54896445
Polymorphism and mutation databases Cosmic; 974319
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number25